These floxed mutant mice possess loxP sites flanking exon 2 of the Camk2a gene. This strain may be useful for generating conditional mutations in applications related to neurological studies.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Camk2a | calcium/calmodulin-dependent protein kinase II alpha |
Mice homozygous for this "falpha-CaMKII" allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exon 2 of the endogenous gene. When bred to Cre-recombinase expressing mice, exon 2 is deleted in the resulting offspring dependent on the tissue specificity of the promoter of the cre transgene.
For example, when crossed with transgenic mice expressing Cre-recombinase in hippocampal CA3 pyramidal cells (see Stock No. 006474), the resulting offspring show altered neurotransmitter release. These "floxed" mice may be useful for neurological studies such as calcium/calmodulin-dependent protein kinase activity.
A targeting vector was designed to insert a loxP site into intron 1 and the loxP- and FRT-flanked LFNT (pgk-neo and hsv-tk) cassette into intron 2 of the endogenous gene. The construct was electroporated into C57BL/6-derived embryonic stem (ES) cells, which were transiently transfected to remove the LFNT selection cassette. Correctly targeted ES cells (with loxP sites flanking exon 2 and a single remaining FRT site) were injected into BALB/c blastocysts. The resulting chimeric mice were bred to C57BL/6 to establish the colony and maintained by breeding homozygous mice together for many generations prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Vadim Y Bolshakov |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | falpha-CaMKII KO |
Gene Symbol and Name | Camk2a, calcium/calmodulin-dependent protein kinase II alpha |
Gene Synonym(s) | |
Strain of Origin | C57BL/6 |
Chromosome | 18 |
Molecular Note | A loxP site was introduced into intron 1 and an frt/loxP-flanked neo cassette was introduced to intron 2 via homologous recombination. The neo cassette was removed by transient expression of flp recombinase in correctly targeted ES cells. Resulting mutant mice carry a floxed exon 2 allele. |
Mutations Made By | Dr. Susumu Tonegawa, Massachusetts Institute of Technology |
When maintaining a live colony, homozygous mice are bred.
When using the CA3 α-CaMKII KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006575 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Camk2a<tm1Vyb> |
Frozen Mouse Embryo | C57BL/6-Camk2a<tm1Vyb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Camk2a<tm1Vyb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Camk2a<tm1Vyb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Camk2a<tm1Vyb>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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