These floxed mutant mice possess loxP sites flanking exon 2 of the Camk2a gene. This strain may be useful for generating conditional mutations in applications related to neurological studies.
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
Mice homozygous for this "falpha-CaMKII" allele are viable and fertile with no reported neurological abnormalities. These mutant mice have loxP sites flanking exon 2 of the endogenous gene. When bred to Cre-recombinase expressing mice, exon 2 is deleted in the resulting offspring dependent on the tissue specificity of the promoter of the cre transgene.
For example, when crossed with transgenic mice expressing Cre-recombinase in hippocampal CA3 pyramidal cells (see Stock No. 006474), the resulting offspring show altered neurotransmitter release. These "floxed" mice may be useful for neurological studies such as calcium/calmodulin-dependent protein kinase activity.
A targeting vector was designed to insert a loxP site into intron 1 and the loxP- and FRT-flanked LFNT (pgk-neo and hsv-tk) cassette into intron 2 of the endogenous gene. The construct was electroporated into C57BL/6-derived embryonic stem (ES) cells, which were transiently transfected to remove the LFNT selection cassette. Correctly targeted ES cells (with loxP sites flanking exon 2 and a single remaining FRT site) were injected into BALB/c blastocysts. The resulting chimeric mice were bred to C57BL/6 to establish the colony and maintained by breeding homozygous mice together for many generations prior to arrival at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Vadim Y Bolshakov|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||falpha-CaMKII KO|
|Gene Symbol and Name||Camk2a, calcium/calmodulin-dependent protein kinase II alpha|
|Strain of Origin||C57BL/6|
|Molecular Note||A loxP site was introduced into intron 1 and an frt/loxP-flanked neo cassette was introduced to intron 2 via homologous recombination. The neo cassette was removed by transient expression of flp recombinase in correctly targeted ES cells. Resulting mutant mice carry a floxed exon 2 allele.|
|Mutations Made By|| |
Dr. Susumu Tonegawa, Massachusetts Institute of Technology
When maintaining a live colony, homozygous mice are bred.
When using the CA3 α-CaMKII KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006575 in your Materials and Methods section.
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