These congenic mice carry the H2g7 gene along with a number of genomic markers derived from the NOD/ShiLtJ strain (Stock No. 001976).
Dr. Edward Leiter, The Jackson Laboratory
129S1.NOD-H2g7 congenic mice are viable, fertile, normal in size, do not display any gross physical or behavioral abnormalities and are diabetes free. Because targeted mutations frequently are made using 129 ES cells, this strain may be useful for identifying Idds in 129 strains.
Idd1, linked to the major histocompatibility locus, is located on Chr 17. This 129S1 congenic strain, carrying a defined genomic segment of Chr 17 from NOD/LtJ that includes Idd1 (19.5cM) and H2g7 (23cM), was produced by mating mice from 129S1/SvImJ (Stock No. 002448) to NOD/ShiLtJ (Stock No. 001976) for seven generations (N7) with selection for H2g7 by FACS analysis at each generation. Sibs were intercrossed to generate mice homozygous for the NOD genomic segment (5.3cM), D17Mit175 (31571388-31571496) through H2g7 (23cM). In addition, the strain also carries genomic markers D17Mit16 (33207435-33207557), D17Mit21 (33851425-33851562) and D17Mit34 (34352109-34352255) derived from NOD/ShiLtJ origin, while D17Mit213 (16319811-16319934) and D17Mit24 (37090217-37090359) are from 129S1/SvImJ. Genome scans using SNPS markers indicate that the rest of the genome is derived from 129S1/SvImJ.