These congenic mice carry the H2g7 gene along with a number of genomic markers derived from the NOD/ShiLtJ strain (Stock No. 001976).
Dr. Edward Leiter, The Jackson Laboratory
Genetic Background | Generation |
---|---|
002448 129S1/SvImJ |
|
Marker Symbol | Marker Name | |
---|---|---|
Idd1 | insulin dependent diabetes susceptibility 1 | |
H2 | histocompatibility-2, MHC | |
D17Mit175 | DNA segment, Chr 17, Massachusetts Institute of Technology 175 |
129S1.NOD-H2g7 congenic mice are viable, fertile, normal in size, do not display any gross physical or behavioral abnormalities and are diabetes free. Because targeted mutations frequently are made using 129 ES cells, this strain may be useful for identifying Idds in 129 strains.
Idd1, linked to the major histocompatibility locus, is located on Chr 17. This 129S1 congenic strain, carrying a defined genomic segment of Chr 17 from NOD/LtJ that includes Idd1 (19.5cM) and H2g7 (23cM), was produced by mating mice from 129S1/SvImJ (Stock No. 002448) to NOD/ShiLtJ (Stock No. 001976) for seven generations (N7) with selection for H2g7 by FACS analysis at each generation. Sibs were intercrossed to generate mice homozygous for the NOD genomic segment (5.3cM), D17Mit175 (31571388-31571496) through H2g7 (23cM). In addition, the strain also carries genomic markers D17Mit16 (33207435-33207557), D17Mit21 (33851425-33851562) and D17Mit34 (34352109-34352255) derived from NOD/ShiLtJ origin, while D17Mit213 (16319811-16319934) and D17Mit24 (37090217-37090359) are from 129S1/SvImJ. Genome scans using SNPS markers indicate that the rest of the genome is derived from 129S1/SvImJ.
When using the 129-Idd1NOD mouse strain in a publication, please cite the originating article(s) and include JAX stock #006500 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous, 1 pair minimum |
Frozen Mouse Embryo | 129.NOD-(D17Mit175-H2)/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129.NOD-(D17Mit175-H2)/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129.NOD-(D17Mit175-H2)/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | 129.NOD-(D17Mit175-H2)/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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