129.NOD-(D17Mit175-H2)/J

Stock number: 006500
Product name: 129-Idd1^NOD
Strain synonyms: 129.NOD-(D17Mit175-D17Mit34)/J, 129.NOD-Chr17, 129.NOD-H2^g7, 129.NOD-Idd1
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

These congenic mice carry the H2^g7 gene along with a number of genomic markers derived from the NOD/ShiLtJ strain (Stock No. 001976).

Detailed description

129S1.NOD-H2^g7 congenic mice are viable, fertile, normal in size, do not display any gross physical or behavioral abnormalities and are diabetes free. Because targeted mutations frequently are made using 129 ES cells, this strain may be useful for identifying Idds in 129 strains.

Development

Idd1, linked to the major histocompatibility locus, is located on Chr 17. This 129S1 congenic strain, carrying a defined genomic segment of Chr 17 from NOD/LtJ that includes Idd1 (19.5cM) and H2^g7 (23cM), was produced by mating mice from 129S1/SvImJ (Stock No. 002448) to NOD/ShiLtJ (Stock No. 001976) for seven generations (N7) with selection for H2^g7 by FACS analysis at each generation. Sibs were intercrossed to generate mice homozygous for the NOD genomic segment (5.3cM), D17Mit175 (31571388-31571496) through H2^g7 (23cM). In addition, the strain also carries genomic markers D17Mit16 (33207435-33207557), D17Mit21 (33851425-33851562) and D17Mit34 (34352109-34352255) derived from NOD/ShiLtJ origin, while D17Mit213 (16319811-16319934) and D17Mit24 (37090217-37090359) are from 129S1/SvImJ. Genome scans using SNPS markers indicate that the rest of the genome is derived from 129S1/SvImJ.

Allele

MGI accession ID: MGI:96400
Marker symbol: Idd1
Marker name: insulin dependent diabetes susceptibility 1
Marker synonyms: Idd-1
Chromosome: 17

Allele

MGI accession ID: MGI:100877
Marker symbol: D17Mit175
Marker name: DNA segment, Chr 17, Massachusetts Institute of Technology 175
Chromosome: 17

Allele

MGI accession ID: MGI:95894
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Marker synonyms: H-2; MHC-II
Chromosome: 17