B6.Cg-Pdgfra^tm8Sor/EiJ

Stock number: 006492
Product name: PDGFRα^fl
Research areas: Research Tools

Description

This strain carries a floxed allele of the platelet derived growth factor receptor alpha (Pdgfra). When used in conjunction with a strain expressing Cre recombinase in neural crest cells (dorsal neural tube), this strain may be useful in studies of cardiovascular and craniofacial development.

Detailed description

These mice possess loxP sites on either side of exon 1 and exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.

For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or 009107 for example), this mutant mouse strain may be useful in studies of cranial and cardiac neural crest cells.

Development

The targeted mutation (exons 1 through 4 flanked by loxP sites) was developed by Dr. Philippe Soriano at the Fred Hutchinson Cancer Research Center. A PGK-neo cassette was inserted downstream of exon 4. The embryonic stem cell line is not known. The resulting chimeric mice were bred to C57BL/6J mice for 12 generations. Dr. Eva Eicher at The Jackson Laboratory obtained the strain from Dr. Soriano and backcrossed the mice to C57BL/6JEi for 9 generations. In 2006, mice were transferred to The Jackson Laboratory Repository and were backcrossed to C57BL/6J at least one generation. In 2020, this colony was backcrossed to C57BL/6J at least one generation.

Allele

Symbol: Pdgfra^tm8Sor
Name: targeted mutation 8, Philippe Soriano
MGI accession ID: MGI:2449440
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Pdgfra
Marker name: platelet derived growth factor receptor, alpha polypeptide
Chromosome: 5
Strain of origin: Not Specified
Molecular note: A loxP site was introduced upstream of exon 1 and a PGK-neo-loxP cassette was introduced downstream of exon 4 via homologous recombination.