This strain carries a floxed allele of the platelet derived growth factor receptor alpha (Pdgfra). When used in conjunction with a strain expressing Cre recombinase in neural crest cells (dorsal neural tube), this strain may be useful in studies of cardiovascular and craniofacial development.
Dr. Philippe Soriano, Mount Sinai School of Medicine
These mice possess loxP sites on either side of exon 1 and exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
For example, when bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 007807 or 009107 for example), this mutant mouse strain may be useful in studies of cranial and cardiac neural crest cells.
The targeted mutation (exons 1 through 4 flanked by loxP sites) was developed by Dr. Philippe Soriano at the Fred Hutchinson Cancer Research Center. A PGK-neo cassette was inserted downstream of exon 4. The embryonic stem cell line is not known. The resulting chimeric mice were bred to C57BL/6J mice for 12 generations. Dr. Eva Eicher at The Jackson Laboratory obtained the strain from Dr. Soriano and backcrossed the mice to C57BL/6JEi for 9 generations. In 2006, mice were transferred to The Jackson Laboratory Repository and were backcrossed to C57BL/6J at least one generation. In 2020, this colony was backcrossed to C57BL/6J at least one generation.
|Allele Name||targeted mutation 8, Philippe Soriano|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Pdgfra, platelet derived growth factor receptor, alpha polypeptide|
|Strain of Origin||Not Specified|
|Molecular Note||A loxP site was introduced upstream of exon 1 and a PGK-neo-loxP cassette was introduced downstream of exon 4 via homologous recombination.|
|Mutations Made By|| |
Eva Eicher, The Jackson Laboratory
When maintaining a live colony, these mice can be bred as homozygotes.
When using the PDGFRαfl mouse strain in a publication, please cite the originating article(s) and include JAX stock #006492 in your Materials and Methods section.