These floxed mutant mice possess loxP sites flanking exons 9 and 10 of the Abcb7 gene. This strain may be useful for generating conditional mutations in applications related to degenerative nerve diseases.
Mark D Fleming, Children's Hospital Boston
Homozygous mice are viable and fertile with no reported neurological or hematological abnormalities. These mutant mice have loxP sites flanking exons 9 and 10 of the endogenous gene. When bred to Cre recombinase expressing mice, exons 9 and 10 are deleted in the offspring dependent on the tissue specificity of the Cre recombinase expressing parent. The donating investigator reports that the null allele is not transmissible due to an effect on the extraembryonic tissues. This mutant may be useful in studying cytosolic Fe-S cluster assembly and metabolism, Friedreich ataxia, anemia, and hematopoiesis.
When bred to a strain expressing Cre recombinase in liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte iron metabolism.
A targeting vector was designed to insert a loxP site into intron 8 and a loxP-flanked neomycin resistance cassette into intron 10 of the X-linked endogenous gene. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells (with loxP sites flanking exons 9 and 10) were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6J to establish the colony. Mutant mice were backcrossed to C57BL/6J mice for 10 generations prior to arrival at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Mark D Fleming|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Abcb7, ATP-binding cassette, sub-family B (MDR/TAP), member 7|
|Strain of Origin||129S4/SvJae|
|Molecular Note||LoxP sites were inserted into the locus to flank exons 9 and 10. Transient cre expression removed a neomycin resistance cassette included in the vector.|
|Mutations Made By|| |
Mark Fleming, Children's Hospital Boston
The targeted gene is on the X chromosome. When maintaining a live colony, homozygous females are bred to hemizygous males.
When using the Abcb7 floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #006490 in your Materials and Methods section.