These floxed mutant mice possess loxP sites flanking exons 9 and 10 of the Abcb7 gene. This strain may be useful for generating conditional mutations in applications related to degenerative nerve diseases.
Mark D Fleming, Children's Hospital Boston
Homozygous mice are viable and fertile with no reported neurological or hematological abnormalities. These mutant mice have loxP sites flanking exons 9 and 10 of the endogenous gene. When bred to Cre recombinase expressing mice, exons 9 and 10 are deleted in the offspring dependent on the tissue specificity of the Cre recombinase expressing parent. The donating investigator reports that the null allele is not transmissible due to an effect on the extraembryonic tissues. This mutant may be useful in studying cytosolic Fe-S cluster assembly and metabolism, Friedreich ataxia, anemia, and hematopoiesis.
When bred to a strain expressing Cre recombinase in liver (see Stock No. 003574 for example), this mutant mouse strain may be useful in studies of hepatocyte iron metabolism.
A targeting vector was designed to insert a loxP site into intron 8 and a loxP-flanked neomycin resistance cassette into intron 10 of the X-linked endogenous gene. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells (with loxP sites flanking exons 9 and 10) were injected into blastocysts and the resulting chimeric mice were bred to C57BL/6J to establish the colony. Mutant mice were backcrossed to C57BL/6J mice for 10 generations prior to arrival at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Mark D Fleming|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Abcb7, ATP-binding cassette, sub-family B (MDR/TAP), member 7|
|Gene Synonym(s)||AA517758; ABC7; ASAT; ATP-binding cassette 7; AU019072; Abc7; Abc7; Atm1p; EST140535; expressed sequence AA517758; expressed sequence AU019072|
|Strain of Origin||129S4/SvJae|
|Molecular Note||LoxP sites were inserted into the locus to flank exons 9 and 10. Transient cre expression removed a neomycin resistance cassette included in the vector.|
|Mutations Made By|| |
Mark Fleming, Children's Hospital Boston
The targeted gene is on the X chromosome. When maintaining a live colony, homozygous females are bred to hemizygous males.
When using the Abcb7 floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #006490 in your Materials and Methods section.
|Heterozygous or Wild-type for Abcb7<tm1Mdf>|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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