B6;129S6-Lig4^tm1Fwa/Kvm

Stock number: 006482
Product name: LigIV KO
Research areas: Immunology, Inflammation and Autoimmunity Research

Development

A targeting vector containing a loxP flanked neomycin resistance gene was used to disrupt the Lig4 exon. The construct was electroporated into 129S1/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 and maintained as an intercross. This strain was transferred to Dr. Kevin Mills at The Jackson Laboratory and backcrossed to C57BL/6 for an additional three generations. It was cryopreserved in 2007.

Allele

Symbol: Lig4^tm1Fwa
Name: targeted mutation 1, Frederick W Alt
MGI accession ID: MGI:1857732
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Lig4
Marker name: ligase IV, DNA, ATP-dependent
Chromosome: 8
Strain of origin: 129S6/SvEvTac
Molecular note: A neomycin resistance cassette replaced more than 50% of the coding sequences for the gene, removing the nucleotide sequences that encode amino acids 143 - 608 of the protein.