These transgenic mice were created to express airway-specific overexpression of epithelial Na+ channels (ENaC), and are useful in studies of cystic fibrosis.
Wanda K. O'Neal, Univ. of North Carolina at Chapel Hill
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These Scnn1b-transgenic mice overexpress the mouse nonvoltage-gated 1 beta, Scnn1b, under the direction of the rat secretoglobin, family 1A, member 1 (uteroglobin; Clara cell secretory protein) promoter. While the donating investigator reports that most hemizygous transgenic mice (80-90%) on the congenic C57BL/6N background (Stock No. 30949) survive past postnatal day 14, hemizygous mice on the congenic C57BL/6J (Stock No. 006438), B6;C3H mixed (Stock No. 005315), and B6C3Fe hybrid (Stock No. 006176) backgrounds at The Jackson Laboratory exhibit an approximately 40% survival rate to weaning age. Hemizygous mice that do not survive die from airway obstruction asphyxia. Mice exhibit chronic inflammation with neutrophil infiltration, chronic mucus hypersecretion and emphysema. These Scnn1b-transgenic mice may be useful in studies of cystic fibrosis, and are available on different genetic backgrounds such as B6;C3H mixed (Stock No. 005315), B6C3Fe hybrid (Stock No. 006176), C57BL6-congenic (Stock No. 006438), and congenic C57BL/6N (Stock No. 030949).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Importation of this model was supported by The Boomer Esiason Foundation.
A transgenic construct containing the protein coding region of the mouse sodium channel, nonvoltage-gated 1 beta gene (Scnn1b) under the control of the rat secretoglobin, family 1A, member 1 (uteroglobin), Scgb1a1, promoter and SV40 polyadenylation site sequence was injected into fertilized B6C3F1 donor eggs. Founder line 6608 was established, and the strain subsequently was backcrossed to C57BL/6J for 12 generations.
Expressed Gene | Scnn1b, sodium channel, nonvoltage-gated 1 beta, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 6608, Richard Boucher |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | betaENaC-Tg |
Gene Symbol and Name | Tg(Scgb1a1-Scnn1b)6608Bouc, transgene insertion 6608, Richard Boucher |
Gene Synonym(s) | |
Promoter | Scgb1a1, secretoglobin family 1A member 1, rat |
Expressed Gene | Scnn1b, sodium channel, nonvoltage-gated 1 beta, mouse, laboratory |
Strain of Origin | (C3H x C57BL/6)F1 |
Chromosome | 5 |
General Note | A second transgenic line, Tg(Scgb1a1-Scnn1b)6047Bouc, was created in the same genetic background. The phenotypes of the two lines are indistinguishable. Complete histological examination of multiple organs and systems reveals no pathology of organs other than lungs of transgenic mice. |
Molecular Note | Approximately 2330 base pairs of 5' flanking sequence from the rat airway-specific Scgb1a1 (also called the Clara cell secretory protein) gene were inserted into the pTG1 vector upstream of exon 1 (104 base pairs, including the transcription initiation site), intron 1 (947 base pairs) and 10 base pairs of exon 2 from the mouse transthyretin gene; the protein-coding region of the mouse Scnn1b cDNA, inserted just downstream of the transthyretin exon 2 splice acceptor, is followed by the SV40 early-region polyadenylation signal. In situ hybridization with a transgene-specific antisense probe confirmed expression in airway epithelia of the lung. The insertion site was identified by in situ hybridization; it is located on chromosome 5 near the B-C1 chromosome band junction. |
Mutations Made By | Wanda O'Neal, Univ. of North Carolina at Chapel Hill |
When maintaining a live colony, these mice are bred as hemizygotes. While the donating investigator reports that most hemizygous transgenic mice (80-90%) survive past postnatal day 14, hemizygous mice at The Jackson Laboratory exhibit an approximately 40% survival rate to weaning age.
When using the βENaC-Tg mouse strain in a publication, please cite the originating article(s) and include JAX stock #006438 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizyous or Non Carrir for Tg(Scgb1a1-Scnn1b)6608Bouc |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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