Homozygous Figlatm1Dean knock-out female mice are devoid of primordial follicles in the ovary and do not express Zp1, Zp2 or Zp3.
Eva Eicher, The Jackson Laboratory
Heterozygous female and homozygous male mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Female homozygous mice are infertile due to absence of primordial follicles in the ovary. No gene product (mRNA) is detected by RNase protection analysis of total ovarian RNA. This mutant mouse strain may be useful in studies of female infertility.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the transcription and translation start site sequence. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6N blastocysts. The resulting chimeric animals were crossed to CF-1 mice, and then backcrossed to B6Ei for at least 5 generations.
|Allele Name||targeted mutation 1, Jurrien Dean|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Figalphatm; Figalphatm1Nih; Figalphatm2Nih|
|Gene Symbol and Name||Figla, folliculogenesis specific basic helix-loop-helix|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A 0.8 kb genomic fragment containing the transcription and translation start sites was replaced with a neomycin selection cassette. RNAse protection assays on RNA derived from ovaries of female homozygous mice demonstrated that no detectable transcript was present.|
|Mutations Made By|| |
Jurrien Dean, NIH / NIDDK
When maintaining a live colony, heterozygous females and homozygous males are bred. Homozygous females are infertile due to absence of primordial follicles in the ovary.
When using the Figα mouse strain in a publication, please cite the originating article(s) and include JAX stock #006430 in your Materials and Methods section.