These POMC-GFP transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Hemizygous mice are viable and express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) under control of the mouse pro-opiomelanocortin-alpha (Pomc1) promoter. As such, UV light-exposed transgenic brain tissues show GFP fluorescence patterns consistent with the endogenous Pomc1 gene, specifically POMC expressing neurons. The donating investigator reports that hrGFP is more stable and resistant to signal fading compared to other GFP's. The donating investigator reports that transgenic males have smaller seminal vesicles, and breeding transgenic males results in infrequent and very small (<2 pups) litter sizes. When non-transgenic males are crossed with transgenic females, fertility is normal. These POMC-GFP transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.
A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence in-frame with the ATG transcriptional start site of the mouse pro-opiomelanocortin-alpha (Pomc1) gene (this also deleted the first 30 nucleotides of Pomc1). Correctly transformed and targeted bacteria were used to isolate the 150 kb BAC, which was then injected into the pronucleus of fertilized one-cell stage FVB embryos. The resulting offspring were bred to FVB mice. Transgenic "POMC-GFP" mice (founder line 1) were bred to wild-type FVB mice for at least 10 generations prior to arrival at The Jackson Laboratory.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | hrGFP is detected in Pomc1-expressing neurons. |
Allele Name | transgene insertion 1, Bradford B Lowell |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | POMC-GFP; POMC-hrGFP |
Gene Symbol and Name | Tg(Pomc1-hrGFP)1Lowl, transgene insertion 1, Bradford B Lowell |
Gene Synonym(s) | |
Promoter | Pomc, pro-opiomelanocortin-alpha, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | hrGFP is detected in Pomc1-expressing neurons. |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence in-frame with the ATG transcriptional start site of the mouse pro-opiomelanocortin-alpha (Pomc1) gene (this also deleted the first 30 nucleotides of Pomc1). Correctly transformed and targeted bacteria were used to isolate the 150 kb BAC, which was then injected into the pronucleus of fertilized one-cell stage FVB embryos. Transgenic line 1 was subsequently established. When exposed to UV light, brain tissue shows GFP fluorescence patterns consistent with endogenous Pomc1 expression. |
When maintaining a live colony, hemizygous females are bred to wildtype sibling or FVB/NJ males. The donating investigator reports that transgenic males have smaller seminal vesicles, and breeding transgenic males results in infrequent and very small (<2 pups) litter sizes. When non-transgenic males are crossed with transgenic females, fertility is normal.
When using the FVB-Tg(Pomc1-hrGFP)1Lowl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006421 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Pomc1-hrGFP)1Lowl |
Frozen Mouse Embryo | FVB-Tg(Pomc1-hrGFP)1Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Pomc1-hrGFP)1Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Pomc1-hrGFP)1Lowl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Pomc1-hrGFP)1Lowl/J Frozen Embryo | $3373.50 |
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