FVB-Tg(Pomc1-hrGFP)1Lowl/J

Stock number: 006421
Research areas: Neurobiology Research, Research Tools

Description

These POMC-GFP transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.

Detailed description

Hemizygous mice are viable and express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) under control of the mouse pro-opiomelanocortin-alpha (Pomc1) promoter. As such, UV light-exposed transgenic brain tissues show GFP fluorescence patterns consistent with the endogenous Pomc1 gene, specifically POMC expressing neurons. The donating investigator reports that hrGFP is more stable and resistant to signal fading compared to other GFP's. The donating investigator reports that transgenic males have smaller seminal vesicles, and breeding transgenic males results in infrequent and very small (<2 pups) litter sizes. When non-transgenic males are crossed with transgenic females, fertility is normal. These POMC-GFP transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.

Development

A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence in-frame with the ATG transcriptional start site of the mouse pro-opiomelanocortin-alpha (Pomc1) gene (this also deleted the first 30 nucleotides of Pomc1). Correctly transformed and targeted bacteria were used to isolate the 150 kb BAC, which was then injected into the pronucleus of fertilized one-cell stage FVB embryos. The resulting offspring were bred to FVB mice. Transgenic "POMC-GFP" mice (founder line 1) were bred to wild-type FVB mice for at least 10 generations prior to arrival at The Jackson Laboratory.

Allele

Symbol: Tg(Pomc1-hrGFP)1Lowl
Name: transgene insertion 1, Bradford B Lowell
MGI accession ID: MGI:3709191
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Pomc1-hrGFP)1Lowl
Marker name: transgene insertion 1, Bradford B Lowell
Chromosome: UN
Strain of origin: FVB
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: hrGFP is detected in Pomc1-expressing neurons.
Molecular note: A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence in-frame with the ATG transcriptional start site of the mouse pro-opiomelanocortin-alpha (Pomc1) gene (this also deleted the first 30 nucleotides of Pomc1). Correctly transformed and targeted bacteria were used to isolate the 150 kb BAC, which was then injected into the pronucleus of fertilized one-cell stage FVB embryos. Transgenic line 1 was subsequently established. When exposed to UV light, brain tissue shows GFP fluorescence patterns consistent with endogenous Pomc1 expression.