B6.FVB-Tg(Npy-hrGFP)1Lowl/J

Stock number: 006417
Product name: NPY-hrGFP , NPY-GFP
Research areas: Neurobiology Research, Research Tools

Description

These mice express humanized Renilla Green Fluorescent Protein (hrGFP) under control of the mouse Npy promoter. The hrGFP expressed from this transgene is more stable and resistant to signal fading compared to other GFP’s. These transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.

Detailed description

Hemizygous mice are viable and fertile. These mice express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) under control of the mouse neuropeptide Y (Npy) promoter. As such, UV light-exposed transgenic brain tissues show GFP fluorescence patterns consistent with the (Npy) gene. The donating investigator reports that the hrGFP expressed from this transgene is more stable and resistant to signal fading compared to other GFP’s. These transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.

Development

A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence into the translational start site of the mouse neuropeptide Y (Npy) gene. Correctly transformed and targeted bacteria were used to isolate the transgenic construct (called "NPY-GFP"). This NPY-GFP transgene was used for pronuclear injection into fertilized one-cell stage FVB embryos. Transgenic offspring were bred to C57BL/6 mice producing offspring with germline transmission of the transgene (founder line 1). Transgenic mice were backcrossed to C57BL/6 mice for at least 8 generations prior to arrival at The Jackson Laboratory.

Allele

Symbol: Tg(Npy-hrGFP)1Lowl
Name: transgene insertion 1, Bradford B Lowell
MGI accession ID: MGI:3709190
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Npy-hrGFP)1Lowl
Marker name: transgene insertion 1, Bradford B Lowell
Chromosome: UN
Strain of origin: FVB
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: brain tissues in patterns consistent with the neuropeptide Y gene; humanized Renilla Green Fluorescent Protein (hrGFP) is more stable and resistant to signal fading compared to other GFP’s
Molecular note: A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence into the translational start site of the mouse neuropeptide Y (Npy) gene. Correctly transformed and targeted bacteria were used to isolate the ~200 kb transgenic construct (called "NPY-GFP"). This NPY-GFP transgene was used for pronuclear injection into fertilized one-cell stage FVB embryos. Founder line 1 showing germline transmission was established. Brain tissues show GFP fluorescence when exposed to UV light.