These mice express humanized Renilla Green Fluorescent Protein (hrGFP) under control of the mouse Npy promoter. The hrGFP expressed from this transgene is more stable and resistant to signal fading compared to other GFP’s. These transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
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N10F12
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Allele Type |
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Transgenic (Reporter) |
Starting at:
$278.00 Domestic price for female 4-week |
348.51 Domestic price for breeder pair |
Hemizygous mice are viable and fertile. These mice express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) under control of the mouse neuropeptide Y (Npy) promoter. As such, UV light-exposed transgenic brain tissues show GFP fluorescence patterns consistent with the (Npy) gene. The donating investigator reports that the hrGFP expressed from this transgene is more stable and resistant to signal fading compared to other GFP’s. These transgenic mice may be useful for studies of neurobiology, energy metabolism, obesity, seizures, and epilepsy.
A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence into the translational start site of the mouse neuropeptide Y (Npy) gene. Correctly transformed and targeted bacteria were used to isolate the transgenic construct (called "NPY-GFP"). This NPY-GFP transgene was used for pronuclear injection into fertilized one-cell stage FVB embryos. Transgenic offspring were bred to C57BL/6 mice producing offspring with germline transmission of the transgene (founder line 1). Transgenic mice were backcrossed to C57BL/6 mice for at least 8 generations prior to arrival at The Jackson Laboratory.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | brain tissues in patterns consistent with the neuropeptide Y gene; humanized Renilla Green Fluorescent Protein (hrGFP) is more stable and resistant to signal fading compared to other GFP’s |
Allele Name | transgene insertion 1, Bradford B Lowell |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | NPY-GFP; Npy-hrGFP |
Gene Symbol and Name | Tg(Npy-hrGFP)1Lowl, transgene insertion 1, Bradford B Lowell |
Gene Synonym(s) | |
Promoter | Npy, neuropeptide Y, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | brain tissues in patterns consistent with the neuropeptide Y gene; humanized Renilla Green Fluorescent Protein (hrGFP) is more stable and resistant to signal fading compared to other GFP’s |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | A bacterial artificial chromosome (BAC) was modified by inserting a humanized Renilla GFP (hrGFP, Stratagene) sequence into the translational start site of the mouse neuropeptide Y (Npy) gene. Correctly transformed and targeted bacteria were used to isolate the ~200 kb transgenic construct (called "NPY-GFP"). This NPY-GFP transgene was used for pronuclear injection into fertilized one-cell stage FVB embryos. Founder line 1 showing germline transmission was established. Brain tissues show GFP fluorescence when exposed to UV light. |
Mutations Made By | Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard) |
When maintaining a live colony, hemizygous mice can be bred to wildtype siblings or C57BL/6J. Alternatively, transgenic mice may be bred together, although the phenotype of homozygous mice is not known (October 2006).
When using the NPY-hrGFP , NPY-GFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #006417 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Npy-hrGFP)1Lowl |
Frozen Mouse Embryo | B6.FVB-Tg(Npy-hrGFP)1Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Npy-hrGFP)1Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.FVB-Tg(Npy-hrGFP)1Lowl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.FVB-Tg(Npy-hrGFP)1Lowl/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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