These mutant mice may be useful in immunological studies exploring the in vivo role of ERAP1 in optimizing peptides for presentation by MHC class I molecules.
Dr. Luc Van Kaer, Vanderbilt University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Erap1 | endoplasmic reticulum aminopeptidase 1 |
Homozygous mutant mice are viable and fertile. The absence of mRNA in splenocyte and of protein in hepatocyte lysates is confirmed via RT-PCR and immunoblot, respectively. Homozygous mutants display reduced cell surface expression of MHC class Ia and Ib molecules, altered presentation of self- and foreign-antigens, and defective CD8+ T-cell responses against class I-presented antigens. These mutant mice may be useful in immunological studies exploring ERAP1's role in vivo in optimizing peptides for presentation by MHC class I molecules.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to replace a fragment from intron 4 up to the 5' end of exon 6 (which contains the sequence encoding the Zinc-binding motif in the putative active site of the endogenous protein) with a neomycin resistance cassette. The construct was electroporated into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into blastocysts and the resulting chimeric mice were bred to C57BL/6 to generate heterozygotes. These mice were then backcrossed to C57BL/6 for at least 10 generations prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Luc Van Kaer |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ERAP1- |
Gene Symbol and Name | Erap1, endoplasmic reticulum aminopeptidase 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 13 |
Molecular Note | A targeting vector was designed to replace a 600 kb fragment from intron 4 up to the 5' end of exon 6 (which contains the sequence encoding the Zinc-binding motif in the putative active site of the endogenous protein) with a neomycin resistance cassette. RT-PCR verified absence of mRNA in splenocytes and no protein is detected in hepatocytes by immunoblot. |
Mutations Made By | Dr. Luc Van Kaer, Vanderbilt University School of Medicine |
When maintaining a live colony, homozygous mice can be bred together.
When using the B6.129S6-Erap1tm1Luc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006413 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Erap1<tm1Luc> |
Frozen Mouse Embryo | B6.129S6-Erap1<tm1Luc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Erap1<tm1Luc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Erap1<tm1Luc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Erap1<tm1Luc>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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