These Adrb3 knock-out mice have modest increases in fat stores and may be useful in studies of obesity.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Homozygous mice are viable and fertile. No gene product (mRNA) is detected by Northern blot analysis from homozygous brown or white adipose tissue. Homozygous mice have modest increases in fat stores (female more than male). In contrast to control animals, homozygous mice are unresponsive to beta-3 adrenergic receptor (beta3-AR) agonist treatment (no effect on adenylate cyclase activity, lipolysis, or gastro-intestinal motility). These mutant mice may be useful in studies of energy balance, obesity, fat metabolism, cholesterol homeostasis, diabetes, and pharmacological screening of beta3-AR agonists for potential drug treatment.
A targeting vector was designed to replace the portion of the endogenous gene coding for residue 120-222 with a PGK-neo cassette. The construct was injected into FVB/N zygotes and these were transferred into FVB/N pseudopregnant females. Offspring heterozygous for the targeted mutation (founder line B2) were maintained on the FVB/N genetic background and then bred together to create a homozygous colony.
|Allele Name||targeted mutation 1, Bradford B Lowell|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||beta3-AR -|
|Gene Symbol and Name||Adrb3, adrenergic receptor, beta 3|
|Strain of Origin||FVB/N|
|General Note||This allele was generated by direct injection of DNA vectors into FVB/N zygotes. (J:30060)|
|Molecular Note||A 306bp genomic fragment containing the sequences encoding the third through the fifth transmembrane domains was replaced with a neomycin selection cassette. An RNase protection assay demonstrated that the encoded transcript was not detectable in brown adipose tissue of homozygous mice.|
|Mutations Made By|| |
Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard)
When maintaining a live colony, homozygous mice are bred together.
When using the Β3-AR mouse strain in a publication, please cite the originating article(s) and include JAX stock #006402 in your Materials and Methods section.