When these Sim1-Cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequences in Sim1-expressing tissues (including hypothalamus). As such, Sim1-Cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for Sim1-transcription factor activity.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Transgene expression is observed in all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain. When these Sim1-Cre mice are bred with mice containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked sequences in Sim1-expressing tissues (including hypothalamus). As such, Sim1-Cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for Sim1-transcription factor activity.
View cre expression characterization.
Of note, Sim1-Cre mice may also available on a C57BL/6J congenic background (see Stock No. 006451).
A bacterial artificial chromosome (BAC) was modified by inserting a Cre recombinase sequence into the translational start site of the mouse Sim1 gene (removing the first 31 basepairs of Sim1. Correctly transformed and targeted bacteria had the selection cassette transiently removed (leaving a single frt site downstream of the Cre recombinase). The resulting construct, called "Sim1-Cre", was used for pro-nuclear injection into fertilized one-cell stage FVB embryos. Transgenic offspring (founder line 1) were established and maintained on a mixed genetic background (C57BL/6, 129/SvJ, and FVB) prior to arrival at The Jackson Laboratory.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain |
Allele Name | transgene insertion 1, Bradford B Lowell |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Sim1Cre; Sim1-Cre |
Gene Symbol and Name | Tg(Sim1-cre)1Lowl, transgene insertion 1, Bradford B Lowell |
Gene Synonym(s) | |
Promoter | Sim1, single-minded family bHLH transcription factor 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | all areas that endogenously express Sim1, including paraventricular hypothalamus and other parts of the brain |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The cre ATG was inserted via recombineering techniques, into the Sim1 ATG site of a BAC containing 137.1 kb of genomic C57BL/6 Sim1 sequences up to and including exon 8. The inserted cre sequence deleted the first 31 bp of the Sim1 gene. |
Mutations Made By | Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard) |
When maintaining a live colony, hemizygous mice are bred to wildtype siblings.
When using the STOCK Tg(Sim1-cre)1Lowl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006395 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Sim1-cre)1Lowl |
Frozen Mouse Embryo | STOCK Tg(Sim1-cre)1Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Sim1-cre)1Lowl/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Sim1-cre)1Lowl/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Sim1-cre)1Lowl/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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