Overview

Also Known As:Mint triple-floxed

These Mint triple-floxed mice may be useful for generating conditional mutations in applications relating to neurotransmission.

Donating Investigator

Dr. Thomas C. Sudhof, Stanford University School of Medicine

Genetic overview

Genetic Background	Generation

Apba1^tm1Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Apba1	amyloid beta (A4) precursor protein binding, family A, member 1

Apba3^tm1Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Apba3	amyloid beta (A4) precursor protein-binding, family A, member 3

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Apba2	amyloid beta (A4) precursor protein-binding, family A, member 2

Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

Triple homozygous knock-in mice are viable and fertile and and do not display any gross physical or behavioral abnormalities. Expression of all three proteins is normal. When crossed with a cre deleter strain that eradicates protein expression, Apba1/Abcba2 (Apba1/2) double knockout and Apba1/2/3 triple knockout mice exhibit a high percentage of postnatal lethality (only ~20% of the mice survive). Apba1/2 mice are visually indistinguishable from their littermates and display no major alterations in breathing, movements or reaction to stimuli several hours after birth, but fail to nurse and die within 24 hours. Their brains are morphologically and structurally normal. Quantitation of 18 neuronal proteins fails to reveal significant changes. Surviving mice show reduced weight gain and exhibit movement dysfunction. Cultured neurons from triple knock-in neonates show impairments in presynaptic neurotransmitter release after treatment with lentiviral cre. Apba1/3 and Apba2/3 double knockouts created from crosses with a cre deleter strain survive normally.

Development

loxP sites flanking the first coding exon of Apba1, residues 368-416 of the Apba2, and exon 3 of Apba3 (encoding residues 201-249) were incorporated into three targeting vectors which also contained Frt-flanked neomycin resistance cassettes inserted into the respective downstream (Apba1 and Apba2) or upstream (Apba3) introns. The constructs were independently electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. The neomycin cassettes were subsequently excised from all but the Apba1 mutant strain in which the drug selection cassette does not interfere with expression. Compound mutant mice were generated by intercrossing the individual mutant lines.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Ho A; Morishita W; Atasoy D; Liu X; Tabuchi K; Hammer RE; Malenka RC; Sudhof TC. 2006. Genetic analysis of Mint/X11 proteins: essential presynaptic functions of a neuronal adaptor protein family. J Neurosci 26(50):13089-101PubMed: 17167098 MGI: J:115573

View All References

Genetics

Apba1^tm1Sud

Allele Symbol: Apba1^tm1Sud

Allele Name	targeted mutation 1, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)
Gene Symbol and Name	Apba1, amyloid beta (A4) precursor protein binding, family A, member 1
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	19
Molecular Note	Flanking loxP sites were introduced to exon 1 and an frt-flanked neomycin resistance cassette was inserted into the downstream intron via homologous recombination.
Mutations Made By	Dr. Thomas Sudhof, Stanford University School of Medicine

Apba3^tm1Sud

Allele Symbol: Apba3^tm1Sud

Allele Name	targeted mutation 1, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)
Gene Symbol and Name	Apba3, amyloid beta (A4) precursor protein-binding, family A, member 3
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	10
Molecular Note	Exon 3, encoding amino acids 201-249 of the phosphotyrosine binding (PTB) domain, is flanked by loxP sites. A single FRT site, the remnant of an FRT-flanked neomycin resistance cassette (neo) previously removed by crossing mice with the cassette to mice expressing Flp recombinase in the germline, is present in the downstream intron. (Retention of the neo cassette ablates expression of this gene in mice.) Immunoblot analysis of brain homogenates demonstrates that mice with this conditional mutation express the protein at wild-type levels.

Apba2^tm1Sud

Allele Symbol: Apba2^tm1Sud

Allele Name	targeted mutation 1, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)
Gene Symbol and Name	Apba2, amyloid beta (A4) precursor protein-binding, family A, member 2
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	7
Molecular Note	The exon encoding amino acids 368-416 of the phosphotyrosine binding (PTB) domain is flanked by loxP sites. A single FRT site, the remnant of an FRT-flanked neomycin resistance cassette (neo) previously removed by crossing mice with the cassette to mice expressing Flp recombinase in the germline, is present in the downstream intron. (Retention of the neo cassette ablates expression of this gene in mice.) Immunoblot analysis of brain homogenates demonstrates that mice with this conditional mutation express the protein at wild-type levels.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Alzheimer's Disease
Research Tools
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

References

Ho A; Morishita W; Atasoy D; Liu X; Tabuchi K; Hammer RE; Malenka RC; Sudhof TC. 2006. Genetic analysis of Mint/X11 proteins: essential presynaptic functions of a neuronal adaptor protein family. J Neurosci 26(50):13089-101PubMed: 17167098 MGI: J:115573

Additional - Apba1^tm1Sud related

Additional - Apba2^tm1Sud related

Additional - Apba3^tm1Sud related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Apba1
- Standard PCR:Apba3
- Standard PCR:Apba2
- Genotyping resources and troubleshooting
Breeding Considerations

When maintained as a live colony, triple homozygous floxed animals are intercrossed.
- Additional Breeding and Husbandry Support
Citation
When using the Mint triple-floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #006394 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Apba2<tm1Sud>, Homozygous for Apba3<tm1Sud>,Homozygous for Apba1<tm1Sud>

Related Products and Services

Frozen Mouse Embryo	B6;129-Apba2<tm1Sud> Apba3<tm1Sud> Apba1<tm1S Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6;129-Apba2<tm1Sud> Apba3<tm1Sud> Apba1<tm1S Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6;129-Apba2<tm1Sud> Apba3<tm1Sud> Apba1<tm1S Frozen Embryos	$3373.50
Frozen Mouse Embryo	B6;129-Apba2<tm1Sud> Apba3<tm1Sud> Apba1<tm1S Frozen Embryos	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Also Known As:Mint triple-floxed

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Apba1tm1Sud

Allele Symbol: Apba1tm1Sud

Apba3tm1Sud

Allele Symbol: Apba3tm1Sud

Apba2tm1Sud

Allele Symbol: Apba2tm1Sud

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Apba1tm1Sud related

Additional - Apba2tm1Sud related

Additional - Apba3tm1Sud related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Apba1^tm1Sud

Allele Symbol: Apba1^tm1Sud

Apba3^tm1Sud

Allele Symbol: Apba3^tm1Sud

Apba2^tm1Sud

Allele Symbol: Apba2^tm1Sud

Additional - Apba1^tm1Sud related

Additional - Apba2^tm1Sud related

Additional - Apba3^tm1Sud related