These floxed mutant mice possess loxP sites flanking the first coding exon of the Cask gene. This strain may be useful for generating conditional mutations in applications related to synaptic function.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Homozygous floxed mice are viable and fertile, but females do not thrive. The body size of mutants is significantly smaller than littermate controls and they exhibit a slightly increased mortality. Knock-in mice are hypomorphs and protein is expressed at less than 30% of normal levels. Crossing of the floxed mutants with mice expressing cre recombinase in the male germline excises the floxed exon and a neomycin resistance gene cassette to create a complete knockout of the gene. Knockout homozygotes die within a few hours of birth. They exhibit a partially penetrant cleft palate syndrome and increased apoptosis in the thalamus, but display no other major developmental changes or deficits in basic electrical properties of their neurons.
A targeting vector designed to introduce flanking loxP sites around exon 1 and a floxed neomycin resistance cassette to intron 1 was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts.
|Expressed Gene||neo, neomycin resistance, bacterial|
|Site of Expression|
|Allele Name||targeted mutation 1, Thomas C Sudhof|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||CASK knockin|
|Gene Symbol and Name||Cask, calcium/calmodulin-dependent serine protein kinase (MAGUK family)|
|Expressed Gene||neo, neomycin resistance, bacterial|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||The first coding exon of the Cask gene is flanked by loxP sites and a neomycin cassette is inserted into the intron adjacent to the floxed intron. The resulting allele is a hypermorph with Cask expression decreased by ~66%.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
When maintained as a live colony, heterozygous females are crossed with homozygous males or heterozygotes are intercrossed. Homozygous females are viable but fail to thrive.
When using the CASK floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #006382 in your Materials and Methods section.
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