These floxed mutant mice possess loxP sites flanking exon 12 of the Braf gene. This strain may be useful for generating conditional mutations in neurological studies.
Alcino J. Silva, University of California, Los Angeles
Homozygous "floxed B-raf" (B-raff/f) mice are viable and fertile with normal B-raf protein expression. When bred to mice expressing Cre recombinase under the control of a promoter of interest, exon 12 of the targeted gene is deleted in the tissue of interest. These mutant mice may be useful in neurological studies such as Ras/Raf and MEK/ERK signaling, synaptic (neural) plasticity, learning and memory.
For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain may be useful in studies of neuron development.
For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003755), this mutant mouse strain may be useful in studies of extraembryonic mammmalian development.
A targeting vector was designed to place loxP sites on both sides of exon 12 (the first exon that encodes the B-raf kinase domain) of the targeted gene. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were used to generate chimeric males. These mice were maintained by backcrossing on a 129T2/SvEmsJ (Stock No. 002065) inbred genetic background for at least 9 generations prior to arrival at The Jackson Laboratory. After 2018, frozen sperm may be used to fertilize oocytes from 129S1/SvImJ inbred females (Stock No. 002448).
|Allele Name||targeted mutation 1, Alcino J Silva|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Braf, Braf transforming gene|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeting construct was designed to flank exon 12 (exon 9 according to nomenclature in J:29234) with loxP sites. This exon is the first to encode the kinase domain. Its excision would result in a frame shift in the ORF, resulting in a null mutation. Western blot confirmed that insertion of the loxP sites did not affect protein expression levels.|
|Mutations Made By|| |
Rachel Chen, University of California, Los Angeles
When maintaining a live colony, heterozygous or homozygous mice can be bred.
When using the B-raff mouse strain in a publication, please cite the originating article(s) and include JAX stock #006373 in your Materials and Methods section.