A chromosome substitution or consomic strain is an inbred strain with one of its chromosomes replaced by the homologous chromosome of another inbred strain. The C57BL/6J and C3H/HeJ inbred strains were selected for this consomic because they differ in their susceptibility to infectious disease, specifically Chlamydia trachomatis. Consomic strains can accelerate quantitative trait loci identification and mapping.

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This congenic strain carries a dominant resistance allele (Ctrq-2) at the C. trachomatis resistance QTL on Chromosome 3 from the C57BL/6J strain.

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