Mgat5 (mannoside acetylglucosaminyltransferase 5) deficient mice exhibit increased responses to induced immune challenges and delayed tumor progression when bred with oncomice. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, autoimmunity and antitumor immunity.
James W Dennis, Samuel Lunenfeld Research Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Mgat5 | mannoside acetylglucosaminyltransferase 5 |
No gene product (protein) is detected by Western blot analysis and enzyme activity is undetectable. Beta-galactosidase activity mimics endogenous gene expression patterns. Homozygotes exhibit abnormal increased leukocyte infiltration in kidney tissue, which is indicative of kidney autoimmune glomerulonephritis. Induced experimental autoimmune encephalomyelitis (EAE) and induced delayed-type hypersensitivity (DTH) responses are increased in homozygotes. Cultured splenocytes isolated from homozygotes produce 2 fold more IFN-gamma and 2 fold less IL4. Mutant mice exhibit delayed tumor progression when bred with oncomice (for example, when crossed to a polyomavirus middle T antigen expressing transgenic strain). Mice that are homozygous for the targeted mutation are viable, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that homozygous females are not fertile. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, autoimmunity and antitumor immunity.
This strain was transferred from the collection of the Consortium for Functional Glycomics.
A targeting vector containing neomycin resistance and beta-galactosidase genes was used to disrupt exon 1. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting male chimeric animals were crossed to 129 female mice, and then backcrossed to C57BL/6 for 9 generations before arriving at The Jackson Laboratory.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, James W Dennis |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | GnTV-; Mgat5- |
Gene Symbol and Name | Mgat5, mannoside acetylglucosaminyltransferase 5 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 1 |
Molecular Note | A LacZ and neomycin resistance cassette replaced part of exon 1. |
Mutations Made By | Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI |
When maintaining a live colony, these mice can be bred as heterozygous females and homozygous males. Homozygous females are infertile.
When using the B6.129-Mgat5tm1Jwd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006335 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Mgat5<tm1Jwd> |
Frozen Mouse Embryo | B6.129-Mgat5<tm1Jwd>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Mgat5<tm1Jwd>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Mgat5<tm1Jwd>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Mgat5<tm1Jwd>/J Frozen Embryo | $3373.50 |
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