Overview

Also Known As:ROSA26^eGFP-DTA

ROSA26-eGFP-DTA mice have a loxP-flanked STOP cassette preventing expression of diphtheria toxin fragment A (DTA). Exposure to Cre recombinase removes the lox-stop fragment - resulting in ablation of the cre-expressing cells. These ROSA26-eGFP-DTA mice are useful for Cre-inducible deletion of specific groups of cells.

C57BL/6J-congenic ROSA26-eGFP-DTA mice are available as Stock No. 032087.

Donating Investigator

Juan Pedro Martinez-Barbera, UCL Institute of Child Health

Genetic overview

Genetic Background	Generation
	N1F14p+N1F5 (2020-04-28 00:00:00)

Gt(ROSA)26Sor^tm1(DTA)Jpmb

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence)	Gt(ROSA)26Sor	gene trap ROSA 26, Philippe Soriano

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Research Applications

Research Tools
Cell Biology Research
Neurobiology Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

510.00 Domestic price for breeder pair

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Details

Detailed Description

The ROSA26-eGFP-DTA allele has a loxP-flanked STOP sequence (EGFP::PGK-neo::3xpolyA) upstream of the diphtheria toxin A subunit (DTA) gene. Mutant mice display widespread expression of EGFP, but DTA transcription is prevented by the strong transcriptional stop sequence. When bred to mice that express Cre recombinase under the control of a promoter of interest, the floxed-STOP cassette is removed and DTA expression is activated - resulting in the specific ablation of the cre-expressing cells. Homozygous mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The donating investigator reports that some homozygous males are subfertile.

ROSA26-eGFP-DTA mice may be useful on their own as a fluorescent reporter, or in combination with cre-expressing mice to produce conditional deletions of specific groups of cells. ROSA26-eGFP-DTA mice may also have applications in toxicology and protein synthesis research.

For example, when crossed to a strain expressing Cre recombinase in the midbrain and dorsal spinal cord (see Stock No. 007807 or 009107), DTA expression is activated resulting in elimination of the cre-expressing cells, this mutant mouse strain may be useful in studies of midbrain and neural tube development.

For example, when crossed to a strain expressing tamoxifen-inducible Cre recombinase in keratinocytes (see Stock No. 005107), DTA expression is activated resulting in alterations in the location and morphology of KRT7 expressing cells and may be useful in studies of acid reflux.

C57BL/6J-congenic ROSA26-eGFP-DTA mice are available as Stock No. 032087.

Development

A targeting vector was designed placing a loxP-flanked Enhanced Green Fluorescent Protein (EGFP, Clontech), PGK-Neo cassette, and a triple SV40 polyadenylation signal fragment upstream of the diphtheria toxin A subunit (DTA). This construct was inserted into the Gt(ROSA)26Sor locus via electroporation into 129S6/SvEvTac-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric males were bred to C57BL/6J females. The resulting heterozygotes were bred to CD1 mice for 2-3 generations and then made homozygous prior to arrival at The Jackson Laboratory.

Expression Data

Expressed Gene	Dta, Diphtheria toxin A chain,
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	Expression of EGFP is widespread. When bred to Cre recombinase expressing mice, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Ivanova A; Signore M; Caro N; Greene ND; Copp AJ; Martinez-Barbera JP. 2005. In vivo genetic ablation by Cre-mediated expression of diphtheria toxin fragment A. Genesis 43(3):129-35PubMed: 16267821 MGI: J:103417

View All References

Genetics

Gt(ROSA)26Sor^tm1(DTA)Jpmb

Allele Symbol: Gt(ROSA)26Sor^tm1(DTA)Jpmb

Allele Name	targeted mutation 1, Juan Pedro Martinez-Barbera
Allele Type	Targeted (Conditional ready (e.g. floxed), Reporter, Inserted expressed sequence)
Allele Synonym(s)	LSL-R26^DTA; R26^DTA; ROSA26^eGFP-DTA; ROSA26GFP-DTA
Gene Symbol and Name	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Gene Synonym(s)
Expressed Gene	Dta, Diphtheria toxin A chain,
Expressed Gene	GFP, Green Fluorescent Protein,
Site of Expression	Expression of EGFP is widespread. When bred to Cre recombinase expressing mice, the loxP-flanked EGFP and stop sequence are removed, and DTA expression is activated, resulting in the specific ablation of cre-expressing cells.
Strain of Origin	129S/SvEv-Gpi1^c
Chromosome	6
General Note	The time lapse between onset of Cre expression and cell death is estimated to be less than 24 hours.
Molecular Note	A targeting vector contained a conditionally expressed DTA construct downstream of a floxed DNA region containing EGFP, a neo cassette and a triple SV40. An EGFP is upstream of the DTA and is constitutively expressed under the control of the ROSA26 protmoter.
Mutations Made By	Juan Pedro Martinez-Barbera, UCL Institute of Child Health

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Developmental Biology Research
  - Cre-lox System
- Genetics Research
  - Tissue/Cell Markers: Cre-lox System
  - Mutagenesis and Transgenesis: Cre-lox System
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
- Reproductive Biology Research
  - Cre-lox System
- Diabetes and Obesity Research
  - loxP
- Cardiovascular Research
  - Cre-lox System
- Fluorescent Proteins
- Toxicology Research
- Cre-lox System
  - loxP-flanked Sequences
Cell Biology Research
- Protein Processing
Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor^tm1(DTA)Jpmb Tg(KRT14-cre/ERT)20Efu/?
involves: 129S/SvEv * CD-1

digestive/alimentary phenotype

abnormal stomach epithelium morphology
- treatment of mice with tamoxifen results in an expansion of Krt7-expressing cells from their ordered appearance at the squamocolumnar junction to more anterior regions of the proximal stomach; the migrating Krt7+ cells do not have squamous properties

Genotype: Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor^tm1(DTA)Jpmb
either: (involves: 129S/SvEv * C57BL/6) or (involves: 129S/SvEv * CD-1)

normal phenotype

no abnormal phenotype detected
- Normal - homozygotes are viable and fertile with no gross abnormalities

Genotype: Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor⁺ H2az2^{Tg(Wnt1-cre)11Rth}/0
either: (involves: 129S/SvEv * C57BL/6 * CBA) or (involves: 129S/SvEv * C57BL/6 * CBA * CD-1)

embryo phenotype

open neural tube
- at E9.5 and E10.5, the cranial neural tube is open dorsally

nervous system phenotype

open neural tube
- at E9.5 and E10.5, the cranial neural tube is open dorsally

decreased midbrain size
- at E9.5 and E10.5 the brain rostral to the otic vesicle is significantly smaller

absent midbrain-hindbrain boundary
- at E9.5 and E10.5, the isthmus (the constriction of the neural tube at the midbrain hindbrain boundary) is absent

References

Ivanova A; Signore M; Caro N; Greene ND; Copp AJ; Martinez-Barbera JP. 2005. In vivo genetic ablation by Cre-mediated expression of diphtheria toxin fragment A. Genesis 43(3):129-35PubMed: 16267821 MGI: J:103417

Additional - Gt(ROSA)26Sor^tm1(DTA)Jpmb related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Gt(ROSA)26Sor STD
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony at The Jackson Laboratory, homozygous females are bred with heterozygous males.
The donating investigator breeds homozygous females with heterozygous males and reports that some homozygous males may be subfertile, although breeding homozygous mice together has been successful at The Jackson Laboratory in the past.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Heterozygote
Citation
When using the ROSA26^eGFP-DTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #006331 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Breeder Pair

Sex

Genotype

Price

Female
Male

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous or Heterozygous for Gt(ROSA)26Sor<tm1(DTA)Jpmb>, 1 pair minimum

Related Products and Services

Frozen Mouse Embryo	STOCK Gt(ROSA)26Sor<tm1(DTA)Jpmb>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	STOCK Gt(ROSA)26Sor<tm1(DTA)Jpmb>/J Frozen Embryos	$2595.00
Frozen Mouse Embryo	STOCK Gt(ROSA)26Sor<tm1(DTA)Jpmb>/J Frozen Embryos	$3373.50
Frozen Mouse Embryo	STOCK Gt(ROSA)26Sor<tm1(DTA)Jpmb>/J Frozen Embryos	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:ROSA26eGFP-DTA

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Gt(ROSA)26Sortm1(DTA)Jpmb

Allele Symbol: Gt(ROSA)26Sortm1(DTA)Jpmb

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gt(ROSA)26Sortm1(DTA)Jpmb/Gt(ROSA)26Sortm1(DTA)Jpmb Tg(KRT14-cre/ERT)20Efu/?involves: 129S/SvEv * CD-1

digestive/alimentary phenotype

Genotype: Gt(ROSA)26Sortm1(DTA)Jpmb/Gt(ROSA)26Sortm1(DTA)Jpmbeither: (involves: 129S/SvEv * C57BL/6) or (involves: 129S/SvEv * CD-1)

normal phenotype

Genotype: Gt(ROSA)26Sortm1(DTA)Jpmb/Gt(ROSA)26Sor+ H2az2Tg(Wnt1-cre)11Rth/0either: (involves: 129S/SvEv * C57BL/6 * CBA) or (involves: 129S/SvEv * C57BL/6 * CBA * CD-1)

embryo phenotype

nervous system phenotype

References

Additional - Gt(ROSA)26Sortm1(DTA)Jpmb related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Breeder Pair

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Also Known As:ROSA26^eGFP-DTA

Gt(ROSA)26Sor^tm1(DTA)Jpmb

Allele Symbol: Gt(ROSA)26Sor^tm1(DTA)Jpmb

Genotype: Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor^tm1(DTA)Jpmb Tg(KRT14-cre/ERT)20Efu/?
involves: 129S/SvEv * CD-1

Genotype: Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor^tm1(DTA)Jpmb
either: (involves: 129S/SvEv * C57BL/6) or (involves: 129S/SvEv * CD-1)

Genotype: Gt(ROSA)26Sor^tm1(DTA)Jpmb/Gt(ROSA)26Sor⁺ H2az2^{Tg(Wnt1-cre)11Rth}/0
either: (involves: 129S/SvEv * C57BL/6 * CBA) or (involves: 129S/SvEv * C57BL/6 * CBA * CD-1)

Additional - Gt(ROSA)26Sor^tm1(DTA)Jpmb related