These transgenic mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE or tetO) and are an effective tool for generating inducible, tissue-specific, targeted mutants to study cell lineage during development. When bred with another transgenic mouse expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), Cre mediated recombination in the resulting bitransgenic offspring can be regulated with the tetracycline analog, doxycycline.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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N11F11
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Allele Type |
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Transgenic (Recombinase-expressing, Inducible) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing either reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA) under the control of tissue-specific promoters, Cre recombinase expression and Cre-mediated recombination in the appropriate tissues of the bitransgenic offspring can be regulated with the tetracycline analog, doxycycline. This strain represents an effective tool for generating inducible tissue specific-targeted mutants to study cell lineage during development.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing the cre recombinase gene under the control of the tetracycline-responsive promoter element (TRE or tetO), minimal CMV promoter and MT-1 polyadenylation site sequence was injected into mixed C57BL/6 and 129 background embryos. Founder mice were bred to FVB/N animals. Upon arrival at The Jackson Laboratory, these mice were backcrossed to C57BL/6J mice for at least 5 generations.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | widespread, inducible expression of Cre recombinase |
Allele Name | transgene insertion 1, Jeffrey A Whitsett |
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Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | (tetO)7CMVtg; (tetO)7-CMV-Cre; (tetO)7-CMV-Cretg; (tetO)7CMV-Cretg; (tetO)7-Cre; TetOCre; tetO-cre; TRECre |
Gene Symbol and Name | Tg(tetO-cre)1Jaw, transgene insertion 1, Jeffrey A Whitsett |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | widespread, inducible expression of Cre recombinase |
Strain of Origin | 129 and C57BL/6 |
Chromosome | UN |
Molecular Note | The transgene is composed of 7 tetO sites, a minimal CMV promoter, and sequence encoding cre recombinase. |
Mutations Made By | Andras Nagy, Lunenfeld-Tanenbaum Research Institute (LTRI), Mount Sinai Hospital |
When using the (tetO)7-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #006234 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non Carrierfor Tg(tetO-cre)1Jaw |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-cre)1Jaw/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-cre)1Jaw/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-cre)1Jaw/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(tetO-cre)1Jaw/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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