STOCK Tg(Pcp2-cre)1Amc/J

Stock number: 006207
Product name: L7Cre
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

In these transgenic mice the L7/Pcp2 modified promoter directs tissue specific expression of cre in cerebellar Purkinje cells. A co-integrated GFP transgene results in GFP expression in the cerebellum.

Detailed description

Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in parasagittal domains of the cerebellum beginning at embryonic day 17 (E17). At E19, low level expression is observed in the most rostral lobe of cerebellum and expression broadens until all Purkinje cells express the transgene in adult mice. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in deletion of the flanked genome. These transgenic mice may be useful in studies utilizing "Cre-lox" technology, specifically regarding the nervous system, development and patterning of cerebellum, and cerebellar hypotrophy converse of Lhermitte-Duclos.

Important Note: These mice also carry a GFP transgene that co-integrated with the Tg(Pcp2-cre)1Amc transgene. As such, GFP expression is reported in the cerebellum.

Development

A targeting vector was designed to place a cre recombinase gene under control of a modified mouse Purkinje cell protein 2 (L7) promoter enhancer (from the L7-deltaAUG vector; all upstream ATG's mutated). Transgenic mice were generated on a Black Swiss background and were bred with C57BL/6 mice to obtain hemizygous mice on this mixed background. These mice were not backcrossed and maintained by breeding transgenic carriers prior to arrival at The Jackson Laboratory.

Important Note: These mice also carry a GFP transgene that co-integrated with the Tg(Pcp2-cre)1Amc transgene. As such, GFP expression is reported in the cerebellum.

Allele

Symbol: Tg(Pcp2-cre)1Amc
Name: transgene insertion 1, Andrew P McMahon
MGI accession ID: MGI:3656031
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Pcp2-cre)1Amc
Marker name: transgene insertion 1, Andrew P McMahon
Chromosome: UN
Strain of origin: Black Swiss
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: parasagittal domains of the cerebellum beginning at embryonic day 17 (E17); at E19, low level expression is observed in the most rostral lobe of cerebellum and expression broadens until all Purkinje cells express the transgene in adult mice.
Molecular note: The transgene was generated by placing the L7-deltaAUG promoter enhancer (from the L7-deltaAUG vector in which all upstream ATGs have been mutated) upstream of cre in the pSP-13Cre plasmid. The L7/Pcp2 modified promoter can direct tissue specific expression of genes downstream of it but translation can only be initiated from the ATG of the cre gene downstream of the promoter, resulting in tissue specific expression of cre protein in cerebellar Purkinje cells. (NOTE: A GFP transgene co-integrated with the Tg(Pcp2-cre)1Amc transgene. As such, GFP expression is reported in the cerebellum [personal communication])