Inactivation of tankyrase 2 in these knock-out mice does not result in detectable alteration in telomere length when monitored through multiple generations of breeding, but homozygous mice have significantly decreased body weight.
Richard J Hodes, NCI/NIH
Mice that are homozygous for the targeted mutation are viable and fertile. No gene product (mRNA) is detected by RT-PCR analysis of activated spleen lymphocytes. While neither telomere shortening nor chromosomal abnormalities (even across multiple generations) are observed, homozygous mice have significantly decreased body weight. These mutant mice may be useful in studies of both telomerase function and telomerase-independent pathways which affect development and metabolism.
A targeting vector was designed to place a loxP-flanked neomycin resistance cassette immediately upstream of exon 1 of the endogenous gene, as well as insert a third loxP site downstream of exon 1. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Mice carrying the "floxed" allele were then bred together to produce homozygotes (TANK2flox/flox) and next bred to C57BL/6 mice carrying the EIIa-Cre transgene to remove exon 1 (leaving a single loxP site). Mice bearing this "TANK2 exon 1-deleted" allele were backcrossed to C57BL/6J for six generations (and selected againt the EIIa-Cre transgene) prior to arrival at The Jackson Laboratory.
|Allele Name||targeted mutation 1.1, Y Jeffrey Chiang|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Tnks2, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||Exon 1 was removed via cre-mediated excision. RT-PCR failed to detect transcript in mutants.|
|Mutations Made By|| |
Jeffrey Chiang, NCI/NIH
When maintaining a live colony, heterozygous mice may be bred together.
When using the TANK2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006200 in your Materials and Methods section.