B6.129S4-Matk^tm1Sor/J

Stock number: 006198
Product name: Matk/CHK KO
Research areas: Cell Biology Research, Developmental Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Homozygous Matk^tm1Sor mice have an approximately 2-fold increase in the primitive hematopoietic stem cell population.

Detailed description

Homozygotes are viable and fertile with no behavioral abnormalities. The donating investigator reports homozygous mutants have no endogenous protein expression. Homozygous mice have an approximately 2-fold increase in the primitive hematopoietic stem cell population SPKLS (c-Kit⁺, Lin^-, Sca-1⁺ in combination with side population cells). Homozygous deficiency also leads to the hyperproliferation of pre-B cells in the presence of Interleukin-7, and impaired IFN-gamma production in lymph and spleen cells upon in vivo antigen challenge. These mutant mice may be useful in studying tyrosine phosphorylation of hematopoietic cells, primitive/early hematopoietic populations, immune cell signaling, and regulation of immunological responses.

Development

A targeting vector was designed to replace exon 7 of the endogenous gene [encoding the tyrosine kinase domain/ATP binding domain] with a neomycin resistance cassette. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred with C57BL/6J mice. The resulting heterozygous offspring were bred to C57BL/6J mice for more than 11 generations prior to being sent to to NCI-Frederick. There, mice were maintained by brother-sister matings prior to sending to The Jackson Laboratory.

Allele

Symbol: Matk^tm1Sor
Name: targeted mutation 1, Philippe Soriano
MGI accession ID: MGI:3526800
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Matk
Marker name: megakaryocyte-associated tyrosine kinase
Chromosome: 10
Strain of origin: 129S4/SvJaeSor
Molecular note: A DNA fragment of approximately 500 bp that includes exon 7, which encodes a peptide sequence essential for ATP binding, has been replaced by a neomycin resistance cassette.