Homozygous Matktm1Sor mice have an approximately 2-fold increase in the primitive hematopoietic stem cell population.
Akira Imamoto, The University of Chicago
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Matk | megakaryocyte-associated tyrosine kinase |
Homozygotes are viable and fertile with no behavioral abnormalities. The donating investigator reports homozygous mutants have no endogenous protein expression. Homozygous mice have an approximately 2-fold increase in the primitive hematopoietic stem cell population SPKLS (c-Kit+, Lin-, Sca-1+ in combination with side population cells). Homozygous deficiency also leads to the hyperproliferation of pre-B cells in the presence of Interleukin-7, and impaired IFN-gamma production in lymph and spleen cells upon in vivo antigen challenge. These mutant mice may be useful in studying tyrosine phosphorylation of hematopoietic cells, primitive/early hematopoietic populations, immune cell signaling, and regulation of immunological responses.
A targeting vector was designed to replace exon 7 of the endogenous gene [encoding the tyrosine kinase domain/ATP binding domain] with a neomycin resistance cassette. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred with C57BL/6J mice. The resulting heterozygous offspring were bred to C57BL/6J mice for more than 11 generations prior to being sent to to NCI-Frederick. There, mice were maintained by brother-sister matings prior to sending to The Jackson Laboratory.
Allele Name | targeted mutation 1, Philippe Soriano |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Matk/CHK- |
Gene Symbol and Name | Matk, megakaryocyte-associated tyrosine kinase |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 10 |
Molecular Note | A DNA fragment of approximately 500 bp that includes exon 7, which encodes a peptide sequence essential for ATP binding, has been replaced by a neomycin resistance cassette. |
Mutations Made By | Akira Imamoto, The University of Chicago |
When maintaining a live colony, these mice are bred as homozygotes.
When using the Matk/CHK KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006198 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Matk<tm1Sor> |
Frozen Mouse Embryo | B6.129S4-Matk<tm1Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Matk<tm1Sor>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Matk<tm1Sor>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Matk<tm1Sor>/J Frozen Embryo | $3373.50 |
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