B6.129P2-Tbxas1^tm1Swl/J

Stock number: 006184
Product name: TXAS KO
Research areas: Cardiovascular Research, Hematological Research, Research Tools

Description

Homozygous Tbxas1^tm1Swl mice exhibit a mild hemostatic defect and lack of arachidonate-induced shock and death.

Detailed description

Homozygotes are viable and fertile with no gross physical or behavioral abnormalities. Northern blot show absence of a full length transcript in homozygous spleen and thymus. Homozygous mice exhibit prolonged bleeding time, defective platelet aggregation, and are protected from arachidonic acid induced-shock. These mice may be useful in studies of vascular biology, including hemostasis and thrombosis, as well as human TXAS-deficiency.

Development

A targeting vector was designed to replace the distal portion exon 9 of the endogenous gene with a phosphoglycerate kinase-hypoxanthine phosphoribosyltransferase (PGK-HPRT) cassette (transcribed in the reverse orientation). This construct was electroporated into 129P2/OlaHsd-derived E14TG2a (HPRT-) embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric mice were bred with C57BL/6J for 5 generations, then intercrossed to homozygosity prior to arrival at The Jackson Laboratory.

Allele

Symbol: Tbxas1^tm1Swl
Name: targeted mutation 1, Shu-Wha Lin
MGI accession ID: MGI:3052866
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tbxas1
Marker name: thromboxane A synthase 1, platelet
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Molecular note: Sequence encoding amino acids 234-333 and the first 233 bp of intron 9 were replaced with an HPRT cassette. Southern blot and PCR using tail DNA confirmed recombination. Northern blot and RT-PCR showed lack of wild-type transcript in spleen and thymus tissues of mutants.