The ENU induced mutation in these mice causes generalized, tonic-clonic seizures or maximal tonic hindlimb extension seizures in response to transcorneal electrical stimulation.Read More +
Mice carrying this dominant missense point mutation in the thymoma viral proto-oncogene 3 (Akt) gene exhibit a low seizure threshold in response to electroconvulsive threshold testing or pentylenetetrazol (PTZ) administration, sporadic tonic-clonic seizures, brain enlargement and ecotopic neurons in the dentate hilus and molecular layer of the hippocampus. This mutant mouse strain may be useful in studies of AKT signalling and epilepsy.
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for exhibiting a low threshold in the electroconvulsive test. The mutation results in an A to T change at codon 219 altering the corresponding amino acid from aspartic acid to valine at position 219 (D219V)in the highly conserved kinase domain. This mutation was maintained on a C57BL/6J background in the laboratory of Dr. Wayne Frankel. Mice were cryopreserved in 2006.
|Allele Name||neuroscience mutagenesis facility, 350|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||Akt3, thymoma viral proto-oncogene 3|
|Gene Synonym(s)||AI851531; D930002M15Rik; D930002M15Rik; MPPH; MPPH2; Nmf350; Nmf350; PKB gamma; PKB-GAMMA; PKBG; PRKBG; Pkbg; RAC-PK-gamma; RAC-gamma; RIKEN cDNA D930002M15 gene; STK-2; expressed sequence AI851531; neuroscience mutagenesis facility, 350|
|Strain of Origin||C57BL/6J|
|Molecular Note||ENU mutagenesis induced an A to T transversion in codon 219 that results in an amino acid substitution of valine for aspatic acid at position 219 (D219V).|
|Heterozygous or wildtype for Akt3<Nmf350>|
A molecular assay to genotype this strain is not available. We will fulfill your order by providing at least two untested males and two untested females (two pairs). The total number, sex, and genotypes will vary, although typically 8 or more mice are provided. Untested animals typically are available to ship between 10 and 14 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing may be required – If recovered animals do not display a phenotype, progeny testing will be required. This testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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