The ENU induced mutation in these mice causes generalized, tonic-clonic seizures or maximal tonic hindlimb extension seizures in response to transcorneal electrical stimulation.
The Jackson Laboratory cannot guarantee that cryorecovery of strains from the discontinued NIH-funded Neuroscience Mutagenesis Facility (NMF) will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.Read More +
Mice carrying this dominant missense point mutation in the thymoma viral proto-oncogene 3 (Akt) gene exhibit a low seizure threshold in response to electroconvulsive threshold testing or pentylenetetrazol (PTZ) administration, sporadic tonic-clonic seizures, brain enlargement and ecotopic neurons in the dentate hilus and molecular layer of the hippocampus. This mutant mouse strain may be useful in studies of AKT signalling and epilepsy.
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males in the Neuroscience Mutagenesis facility at The Jackson Laboratory. Mutagenized males were crossed to C57BL/6J females; G3 descendants of the mutagenized males were selected for exhibiting a low threshold in the electroconvulsive test. The mutation results in an A to T change at codon 219 altering the corresponding amino acid from aspartic acid to valine at position 219 (D219V)in the highly conserved kinase domain. This mutation was maintained on a C57BL/6J background in the laboratory of Dr. Wayne Frankel. Mice were cryopreserved in 2006.
|Allele Name||neuroscience mutagenesis facility, 350|
|Allele Type||Chemically induced (ENU)|
|Gene Symbol and Name||Akt3, thymoma viral proto-oncogene 3|
|Gene Synonym(s)||AI851531; D930002M15Rik; D930002M15Rik; MPPH; MPPH2; Nmf350; Nmf350; PKB gamma; PKB-GAMMA; PKBG; PRKBG; Pkbg; RAC-PK-gamma; RAC-gamma; RIKEN cDNA D930002M15 gene; STK-2; expressed sequence AI851531; neuroscience mutagenesis facility, 350|
|Strain of Origin||C57BL/6J|
|Molecular Note||ENU mutagenesis induced an A to T transversion in codon 219 that results in an amino acid substitution of valine for aspatic acid at position 219 (D219V).|
|Please inquire about possible genotypes.|
At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical).
The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to
ship between 10 and 14 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery
will be done, extending the overall recovery time to approximately 25 weeks. Progeny testing is required to identify the
genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the
recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of
interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny
testing at your facility. If you perform the progeny testing, there is no guarantee that a carrier will be identified.
If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female
(one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains
requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some
strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the
time taken to produce the mice identified as carrying the mutation. Please Customer Service for more information
on the cost of progeny testing for a strain.
Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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