B6.129S2(C)-Itgae^tm1Cmp/J

Stock number: 006144
Product name: _αE^-
Research areas: Cell Biology Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

Homozygous null mice exhibit reduced numbers of intestinal intraepithelial lymphocytes and lamina propria lymphocytes. These mice may be useful in studies of the immune system, including T cell function of mucosal T lymphocytes.

Detailed description

Mice that are homozygous for this targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No endogenous gene expression is detected in intraepithelial lymphocytes by FACS analysis or on TGF-beta1-treated splenocytes by immunoprecipitation. Homozygous null mice exhibit reduced numbers of intestinal intraepithelial lymphocytes and lamina propria lymphocytes. These mice may be useful in studies of the immune system, including T cell function.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector containing a neomycin resistance gene was used to replace exon 10 of the endogenous gene. The construct was electroporated into the 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric mice were bred to BALB/c to generate mutant colonies. After, mutant mice were backcrossed to C57BL/6 for more than 10 generations before arriving at The Jackson Laboratory.

These mice were provided to The Jackson Laboratory by Dr. Gregg A. Hadley of The University of Maryland with permission from Dr. Christina M. Parker from The Dana-Farber Cancer Institute.

Allele

Symbol: Itgae^tm1Cmp
Name: targeted mutation 1, Christina M Parker
MGI accession ID: MGI:2136817
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Itgae
Marker name: integrin alpha E, epithelial-associated
Chromosome: 11
Strain of origin: 129S2/SvPas
Molecular note: Replacement of exon 10 with a neomycin resistance gene. No Itgae expression is detected in intraepithelial lymphocytes (IEL) by FACS analysis.