Homozygous null mice exhibit reduced numbers of intestinal intraepithelial lymphocytes and lamina propria lymphocytes. These mice may be useful in studies of the immune system, including T cell function of mucosal T lymphocytes.
Gregg A. Hadley, U. Maryland Baltimore
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Itgae | integrin alpha E, epithelial-associated |
Mice that are homozygous for this targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. No endogenous gene expression is detected in intraepithelial lymphocytes by FACS analysis or on TGF-beta1-treated splenocytes by immunoprecipitation. Homozygous null mice exhibit reduced numbers of intestinal intraepithelial lymphocytes and lamina propria lymphocytes. These mice may be useful in studies of the immune system, including T cell function.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a neomycin resistance gene was used to replace exon 10 of the endogenous gene. The construct was electroporated into the 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts. The resulting chimeric mice were bred to BALB/c to generate mutant colonies. After, mutant mice were backcrossed to C57BL/6 for more than 10 generations before arriving at The Jackson Laboratory.
These mice were provided to The Jackson Laboratory by Dr. Gregg A. Hadley of The University of Maryland with permission from Dr. Christina M. Parker from The Dana-Farber Cancer Institute.
Allele Name | targeted mutation 1, Christina M Parker |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | alphaE-; CD103- |
Gene Symbol and Name | Itgae, integrin alpha E, epithelial-associated |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 11 |
Molecular Note | Replacement of exon 10 with a neomycin resistance gene. No Itgae expression is detected in intraepithelial lymphocytes (IEL) by FACS analysis. |
Mutations Made By | Dr. Christina Parker, Dana-Farber Cancer Institute |
When maintaining a live colony, these mice are bred as homozygotes.
When using the αE- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006144 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Itgae<tm1Cmp> |
Frozen Mouse Embryo | B6.129S2(C)-Itgae<tm1Cmp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2(C)-Itgae<tm1Cmp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2(C)-Itgae<tm1Cmp>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S2(C)-Itgae<tm1Cmp>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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