FVB/N-Tg(Thy1-cre)1Vln/J

Stock number: 006143
Research areas: Mouse/Human Gene Homologs, Neurobiology Research, Research Tools

Description

Cre expression is driven in neurons of the postnatal cortex and hippocampus of hemizygous mice. These mice may be useful in studies of the nervous system, including Alzheimer's disease.

Detailed description

Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is observed in nearly all neurons in cortex and hippocampus. When bred with any mouse containing a loxP-flanked sequence of interest, Cre-mediated recombination will result in postnatal, neuron-specific deletion of the flanked genome. These mice may be useful in studies of the nervous system, including Alzheimer's disease.

View cre expression characterization.

Development

A recombinant vector was designed to place cre recombinase cDNA under control of the mouse Thy1 gene promoter (Thy1.2 sequence). This construct was microinjected into FVB/N pre-nuclear embryos (0.5 day old). A selected founder was bred to FVB/N mice and backcrossed to the same for more than 7 generations. These mice, originally developed by Dr. Fred Van Leuven of The Katholieke Universiteit (Leuven, Belgium) were sent to The Jackson Laboratory from GIGA Transgenics Facility (Liege, Belgium) as hemizygotes in 2006. Upon arrival, mutant mice were bred to FVB/NJ mice for one generation and are now maintained by breeding transgenic mice with FVB/NJ or wildtype siblings.

Allele

Symbol: Tg(Thy1-cre)1Vln
Name: transgene insertion 1, Fred Van Leuven
MGI accession ID: MGI:2684620
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Thy1-cre)1Vln
Marker name: transgene insertion 1, Fred Van Leuven
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: neurons of the postnatal cortex and hippocampus
Molecular note: Sequence encoding cre recombinase was adjoined to the mouse Thy1 promoter. Expression was driven in neurons of the postnatal cortex and hippocampus.