Mice homozygous for this targeted mutation exhibit an increase in the number of circulating white blood cells with monocytes and eosinophils having the largest percent of increase. There is considerable hyperplasia of the various epithelial cell lineages. Mutant mice may be useful in studies of inflammatory responses in the lungs, eye, and skin, angiogenesis and vascular pathophysiology, cancer, chemotherapy, apoptosis, and cell differentiation and migration.
Jack Lawler, Beth Israel Deaconess Medical Center
Mice homozygous for this targeted mutation are viable and fertile, with an approximate 20% decrease in embryo/neonate viability and a mild and variable lordotic curvature of the spine apparent from birth. Homozygous mice have an abnormal, but no full length transcript in multiple tissues. Western analysis confirmed the absence of the protein in platelets. Homozygotes exhibit an increase in the number of circulating white blood cells. During the first four to ten weeks of life, homozygotes exhibit patches of acute and organizing pneumonia. At later time points, there is considerable hyperplasia of the various epithelial cell lineages. Mutant mice also have an increased number of retinal endothelial cells and inappropriate remodeling and maturation of retinal vasculature following injury. On the FVB/N background, spontaneous tumor growth and vasculature are significantly increased compared to wildtype. Mutant mice may be useful in studies of inflammatory responses in the lungs, eye, and skin, angiogenesis and vascular pathophysiology, cancer, chemotherapy, apoptosis, and cell differentiation and migration.
A targeting vector was designed to replace exon 2, intron 3, and exon 3 of the endogenous gene with a phosphoglycerate kinase–neomycin resistance cassette (PGK-neo) cassette. This construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. Chimeric males were bred with C57BL/6J females. Mutant mice were backcrossed to C57BL/6J for 8 generations prior to sending to The Jackson Laboratory.
|Allele Name||targeted mutation 1, Richard Hynes|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||TSP-1; TSP-1-; TSP1-; Thbs1-; Tsp-1KO; tbsp1-|
|Gene Symbol and Name||Thbs1, thrombospondin 1|
|Gene Synonym(s)||THBS; THBS-1; TSP; TSP-1; TSP1; Thbs-1; Thbs-1; Tsp1; tbsp1|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin selection cassette replaced exon 2, intron 3, and exon 3. An RNase protection assay demonstrated that no detectable transcript was present in multiple tissues of homozygous animals. Western analysis confirmed the absence of the encoded protein.|
|Mutations Made By|| |
Jack Lawler, Beth Israel Deaconess Medical Center
When maintaining a live colony, these mice can be bred as homozygotes. Of note, homozygous matings result in fewer litters and an approximate 20% decrease in embryo/neonate viability.
When using the TSP1- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006141 in your Materials and Methods section.
|Heterozygous for Thbs1<tm1Hyn>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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