These transgenic mice express the luciferase gene driven by two copies of the NF-κB regulatory element, which allow identification of NF-κB transcriptional activity in any tissue.
Dr. Mercedes Rincon, The University of Vermont
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Not Applicable | H2 | histocompatibility-2, MHC |
Allele Type |
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Transgenic (Reporter) |
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mutant mice have the luciferase gene driven by two copies of the NF-kappaB (NF-kB or NFkB) regulatory element. The presence of nuclear NF-kB DNA binding activity (as detected by electrophoretic mobility shift assay [EMSA]) is consistent with luciferase reporter activity; these reporter mice identify NF-kB transcriptional activity in any tissue. These transgenic mice may be useful in studies of immunology, cellular signaling, signal transduction, apoptosis, and transcription factor function.
The pBIIX-luciferase (pBIIX-luc) targeting vector contains the firefly luciferase gene (luc) driven by two NF-kB binding sites from the kappa light chain enhancer in front of a minimal fos promoter. This construct was microinjected into (C57BL/6 x SJL)F2 embryos. Transgenic offspring (founder line 26) were crossed with B10.BR-H2k2 H2-T18a/SgSnJ mice (Stock No. 000465) for approximately 20 generations before arriving at The Jackson Laboratory.
Expressed Gene | luc, luciferase, firefly |
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Site of Expression | Luciferase reporter activity identifies NF-kB transcriptional activity in any tissue. |
Allele Name | k variant |
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Allele Type | Not Applicable |
Allele Synonym(s) | H-2k |
Gene Symbol and Name | H2, histocompatibility-2, MHC |
Gene Synonym(s) | |
Site of Expression | Cell surface of almost all nucleated cells. |
Strain of Origin | Not Applicable |
Chromosome | 17 |
General Note | The k variant has been observed in the following strains: C3H, C3H/HeJ, C3H/An, C3H/Bi, C3H/DiSn, C3H/St, CBA, CBA/CaJ, CBA/N, AKR/J, CE/J, C57BR/cdJ, C58/J, MA/J, RF/J, ST/bJ, MRL/MpJ-Faslpr/J |
Allele Name | transgene insertion 26, Mercedes Rincon |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(NFkB/Fos-luc)26Rinc, transgene insertion 26, Mercedes Rincon |
Gene Synonym(s) | |
Promoter | Fos, FBJ osteosarcoma oncogene, mouse, laboratory |
Promoter | Nfkb1, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105, mouse, laboratory |
Expressed Gene | luc, luciferase, firefly |
Site of Expression | Luciferase reporter activity identifies NF-kB transcriptional activity in any tissue. |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | The pBIIX-luciferase (pBIIX-luc) transgenic construct contains the firefly luciferase gene (luc) driven by two NF-kB binding sites from the kappa light chain enhancer in front of a minimal fos promoter. |
Mutations Made By | Dr. Mercedes Rincon, The University of Vermont |
When maintaining a live colony, these mice are bred as hemizygotes. According to the donating investigator, homozygotes are not viable.
When using the NFkB-luc mouse strain in a publication, please cite the originating article(s) and include JAX stock #006100 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(NFkB/Fos-luc)26Rinc |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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