B10.Cg-H2^k Tg(NFkB/Fos-luc)26Rinc/J

Stock number: 006100
Product name: NFkB-luc
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These transgenic mice express the luciferase gene driven by two copies of the NF-κB regulatory element, which allow identification of NF-κB transcriptional activity in any tissue.

Detailed description

Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Mutant mice have the luciferase gene driven by two copies of the NF-kappaB (NF-kB or NFkB) regulatory element. The presence of nuclear NF-kB DNA binding activity (as detected by electrophoretic mobility shift assay [EMSA]) is consistent with luciferase reporter activity; these reporter mice identify NF-kB transcriptional activity in any tissue. These transgenic mice may be useful in studies of immunology, cellular signaling, signal transduction, apoptosis, and transcription factor function.

Development

The pBIIX-luciferase (pBIIX-luc) targeting vector contains the firefly luciferase gene (luc) driven by two NF-kB binding sites from the kappa light chain enhancer in front of a minimal fos promoter. This construct was microinjected into (C57BL/6 x SJL)F2 embryos. Transgenic offspring (founder line 26) were crossed with B10.BR-H2^k2 H2-T18^a/SgSnJ mice (Stock No. 000465) for approximately 20 generations before arriving at The Jackson Laboratory.

Allele

Symbol: Tg(NFkB/Fos-luc)26Rinc
Name: transgene insertion 26, Mercedes Rincon
MGI accession ID: MGI:3618609
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(NFkB/Fos-luc)26Rinc
Marker name: transgene insertion 26, Mercedes Rincon
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: luc,luciferase,firefly
Site of expression: Luciferase reporter activity identifies NF-kB transcriptional activity in any tissue.
Molecular note: The pBIIX-luciferase (pBIIX-luc) transgenic construct contains the firefly luciferase gene (luc) driven by two NF-kB binding sites from the kappa light chain enhancer in front of a minimal fos promoter.

Allele

Symbol: H2^k
Name: k variant
MGI accession ID: MGI:3579322
Generation method: Not Applicable
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Chromosome: 17
Strain of origin: Not Applicable
Site of expression: Cell surface of almost all nucleated cells.
General note: The k variant has been observed in the following strains: C3H, C3H/HeJ, C3H/An, C3H/Bi, C3H/DiSn, C3H/St, CBA, CBA/CaJ, CBA/N, AKR/J, CE/J, C57BR/cdJ, C58/J, MA/J, RF/J, ST/bJ, MRL/MpJ-Fas^lpr/J