These floxed mutant mice possess loxP sites flanking the 3.4-kb upstream regulatory element (URE) of the Spi1 locus. This strain may be useful for generating conditional mutations in applications related to acute myeloid leukemia.
Daniel G. Tenen, Beth Israel Deaconess Medical Center
Mice that are homozygous for the targeted mutation are viable, normal in size, and do not display any gross physical or behavioral abnormalities. Endogenous protein expression is unaffected by the loxP sequences flanking an upstream regulatory region (URE). When bred to mice with a cre recombinase gene under the control of a promoter of interest, the URE of the targeted gene is deleted in the tissue of interest. Deletion of this "floxed" URE may be useful in studying T cell lymphoma, AML and other cancers, as well as transcription factors, hematopoiesis, and development of multiple cell lineages.
|Allele Name||targeted mutation 1.2, Daniel G Tenen|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||-14kb UREloxP (neo-); URE floxed (neo-)|
|Gene Symbol and Name||Spi1, spleen focus forming virus (SFFV) proviral integration oncogene|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|General Note||Crossing mice bearing this "floxed" allele with mice expressing Cre recombinase under control of a selected promoter will result in excision of the URE in the cell/tissue type of interest.|
|Molecular Note||This allele was generated by crossing mice bearing Sfpi1tm1Dgt with mice ubiquitously expressing FLP recombinase to produce progeny in which the FRT-flanked PGKneo cassette has been deleted. This leaves a single FRT site immediately upstream of the 5' member of a pair of loxP sites flanking the 3.4-kb upstream regulatory element (URE) that is located 14 kb upstream of the endogenous gene. Expression of the protein is unaffected by presence of the FRT or loxP sites.|
When maintaining a live colony, these mice are bred as homozygotes.
When using the URE floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #006099 in your Materials and Methods section.