B6.Cg-Msr1^tm1Csk/J

Stock number: 006096
Product name: MSR-
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Macrophages from these Msr1 knock-out mice show a marked decrease in mLDL uptake in vitro and show an increased susceptibility to bacterial infection. These mice may be useful in studies of immunity and host defense, cholesterol transport in macrophages, macrophage-derived foam cells, and atherosclerosis.

Detailed description

Homozygotes are viable and fertile. Liver sections treated with antibodies against type I and type II macrophage scavenger class A receptors show no protein staining. Homozygous mice have less severe lesion development in Apoe-deficient atherosclerosis model. Peritoneal macrophages have reduced mLDL uptake in vitro. Homozygous mice have increased susceptibility to pathogens. These mice may be useful in studies of immunity and host defense, cholesterol transport in macrophages, macrophage-derived foam cells, and atherosclerosis.

Development

A pGK1neo cassette replaces alpha-helical coiled coil sequence of exon 4 (essential for the formation of functional trimeric receptors) of the endogenous gene. The construct was electroporated into “A3-1” embryonic stem (ES) cells of “129 origin.” Correctly targeted ES cells were injected into C57BL/6J blastocysts and transplanted into the uteri of ICR females. The resulting chimeric males were backcrossed to ICR females and heterozygous mice were bred to generate homozygous mice. During or near 1998, Dr. Hiroshi Suzuki and Dr. Tatsuhiko Kodama sent these mutant mice to Dr. Menno de Winther at Maastricht University in The Netherlands. There, mutant mice were backcrossed to C57BL/6JIco for 12 generations before arriving at The Jackson Laboratory.

Allele

Symbol: Msr1^tm1Csk
Name: targeted mutation 1, Chugai Pharmaceutical Co, Ltd
MGI accession ID: MGI:1857874
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Msr1
Marker name: macrophage scavenger receptor 1
Chromosome: 8
Strain of origin: 129X1/SvJ
Molecular note: A neomycin cassette was inserted into the EcoRI site in exon 4, which encodes the alpha helical coiled-coil structure essential for the formation of functional trimeric receptors. Protein was not detectable by immunostaining of liver sections from homozygous mutant animals.