Macrophages from these Msr1 knock-out mice show a marked decrease in mLDL uptake in vitro and show an increased susceptibility to bacterial infection. These mice may be useful in studies of immunity and host defense, cholesterol transport in macrophages, macrophage-derived foam cells, and atherosclerosis.
Menno de Winther, Academic Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Msr1 | macrophage scavenger receptor 1 |
Homozygotes are viable and fertile. Liver sections treated with antibodies against type I and type II macrophage scavenger class A receptors show no protein staining. Homozygous mice have less severe lesion development in Apoe-deficient atherosclerosis model. Peritoneal macrophages have reduced mLDL uptake in vitro. Homozygous mice have increased susceptibility to pathogens. These mice may be useful in studies of immunity and host defense, cholesterol transport in macrophages, macrophage-derived foam cells, and atherosclerosis.
A pGK1neo cassette replaces alpha-helical coiled coil sequence of exon 4 (essential for the formation of functional trimeric receptors) of the endogenous gene. The construct was electroporated into “A3-1” embryonic stem (ES) cells of “129 origin.” Correctly targeted ES cells were injected into C57BL/6J blastocysts and transplanted into the uteri of ICR females. The resulting chimeric males were backcrossed to ICR females and heterozygous mice were bred to generate homozygous mice. During or near 1998, Dr. Hiroshi Suzuki and Dr. Tatsuhiko Kodama sent these mutant mice to Dr. Menno de Winther at Maastricht University in The Netherlands. There, mutant mice were backcrossed to C57BL/6JIco for 12 generations before arriving at The Jackson Laboratory.
Allele Name | targeted mutation 1, Chugai Pharmaceutical Co, Ltd |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | MSR-; Msr1tm1Kod; Msr1-KO; Scara1_; Scvrtm1; SR-; SR-A-; Sra-; SR-AI/II KO; SRAKO; SRKO |
Gene Symbol and Name | Msr1, macrophage scavenger receptor 1 |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 8 |
Molecular Note | A neomycin cassette was inserted into the EcoRI site in exon 4, which encodes the alpha helical coiled-coil structure essential for the formation of functional trimeric receptors. Protein was not detectable by immunostaining of liver sections from homozygous mutant animals. |
Mutations Made By | Menno de Winther, Academic Medical Center |
When maintaining a live colony, these mice are maintained as homozygotes.
When using the MSR- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006096 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Msr1<tm1Csk> |
Frozen Mouse Embryo | B6.Cg-Msr1<tm1Csk>/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Msr1<tm1Csk>/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Msr1<tm1Csk>/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Msr1<tm1Csk>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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