In these Mcl1tm2Sjk knock-out mice portions of Mcl-1 exons 1 and 2 were replaced with an internal ribosome entry site allowing the expression of a neomycin–b-galactosidase fusion protein, but lack detectable expression of MCL-1 protein.
Dr. Stanley J. Korsmeyer, Dana-Farber Cancer Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Mcl1 | myeloid cell leukemia sequence 1 |
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice are embryonic lethal. These mutant mice may be useful in studies of immune function, including apoptosis, B and T cell development, and bone marrow cell differentiation.
When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 006088) and a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126), this mutant mouse strain may be useful in studies of lymphocyte development.
When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 006088) and a strain expressing interferon inducible Cre recombinase in the lymphocytes (Stock No. 003556), this mutant mouse strain may be useful in studies of lymphocyte development.
A targeting vector containing an internal ribosomal entry site (IRES) fused to a neomycin-beta-galactosidase fusion protein (beta-geo) was used to replace a portion from within exon 1 to within exon 2 of the endogenous gene. This construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected in C57BL/6 balstocysts and the resulting chimeric mice were crossed to C57BL/6J. Heterozygotes were backcrossed to C57BL/6J mice for more than 10 generations prior to arrival at The Jackson Laboratory.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 2, Stanley J Korsmeyer |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Mcl-1null; Mcl1-bGeo |
Gene Symbol and Name | Mcl1, myeloid cell leukemia sequence 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | Not Specified |
Chromosome | 3 |
Molecular Note | A sequence fragment extending from within exon 1 through a 5' portion of exon 2 was replaced with an IRES-betageo cassette. Western blot analysis of mutant murine embryonic fibroblasts indicated that no normal protein is produced from this allele. |
Mutations Made By | Dr. Stanley Korsmeyer, Dana-Farber Cancer Institute |
When maintaining a live colony, these mice are bred as heterozygotes. Homozygous mice die in utero.
When using the Mcl-1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #006072 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Mcl1<tm2Sjk> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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