B6.129X1-Mcl1^tm2Sjk/J

Stock number: 006072
Product name: Mcl-1 KO
Research areas: Apoptosis Research, Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

In these Mcl1^tm2Sjk knock-out mice portions of Mcl-1 exons 1 and 2 were replaced with an internal ribosome entry site allowing the expression of a neomycin–b-galactosidase fusion protein, but lack detectable expression of MCL-1 protein.

Detailed description

Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice are embryonic lethal. These mutant mice may be useful in studies of immune function, including apoptosis, B and T cell development, and bone marrow cell differentiation.

When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 006088) and a strain with a Cd19 null allele and expressing Cre recombinase during the B lymphocyte development (Stock No. 004126), this mutant mouse strain may be useful in studies of lymphocyte development.

When bred to a strain with loxP sites inserted into the same targeted allele (Stock No. 006088) and a strain expressing interferon inducible Cre recombinase in the lymphocytes (Stock No. 003556), this mutant mouse strain may be useful in studies of lymphocyte development.

Development

A targeting vector containing an internal ribosomal entry site (IRES) fused to a neomycin-beta-galactosidase fusion protein (beta-geo) was used to replace a portion from within exon 1 to within exon 2 of the endogenous gene. This construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were injected in C57BL/6 balstocysts and the resulting chimeric mice were crossed to C57BL/6J. Heterozygotes were backcrossed to C57BL/6J mice for more than 10 generations prior to arrival at The Jackson Laboratory.

Allele

Symbol: Mcl1^tm2Sjk
Name: targeted mutation 2, Stanley J Korsmeyer
MGI accession ID: MGI:2684150
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Mcl1
Marker name: myeloid cell leukemia sequence 1
Chromosome: 3
Strain of origin: Not Specified
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: A sequence fragment extending from within exon 1 through a 5' portion of exon 2 was replaced with an IRES-betageo cassette. Western blot analysis of mutant murine embryonic fibroblasts indicated that no normal protein is produced from this allele.