Mice homozygous for the targeted mutation are viable, fertile, and do not display any gross anatomical or behavioral abnormalities. FACS analysis of splenocytes confirms homozygous mice do not express the endogenous protein. When stimulated with anti -CD3, splenocytes cultured from homozygous mice show significant early T cell activation defects (proliferation and IL-2 production). When stimulated with anti-TCR and anti-CD28, CD4+ T splenocytes cultured from homozygous mice exhibit mild defects in Th2 cytokine production, but respond normally to cytokine stimulation. These mutant mice may be useful in studies of innate and adaptive immunity, lymphocyte signaling/costimulation, T cell activation, Th1/Th2 cytokine function, and CD2/SLAM family signal transduction.
A targeting vector was designed to replace the 3' portion of exon 2 of the endogenous gene with a neomycin resistance gene. The construct was injected into 129/SvEES embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and next implanted into pseudopregnant surrogate mothers. Chimeric mice were subsequently bred with C57BL/6 mice for one generation before arrival at The Jackson Laboratory. Upon arrival, mutant mice were backcrossed to C57BL/6J for approximately 2 generations.
|Allele Name||targeted mutation 1, David J McKean|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Ly9, lymphocyte antigen 9|
|Strain of Origin||129/Sv|
|General Note||Despite a significant defect in early T cell activation and a slight but significant defect in the Th2 response of CD4+ T cells, homozygous mutant mice mount appropriate T and B cell responses to lymphocytic choriomeningitis virus, their NKT cell development is normal, and mutant macrophages exhibit no defects in cytokine production or bacterial killing.|
|Molecular Note||A 3' part of exon 2 and all of intron 2 have been replaced by a neomycin resistance gene. Western blot analysis of thymocytes and FACS analysis of splenocytes from homozygous mutant mice using rabbit antisera against the extracellular domain detected no LY9 (CD229) protein on these cells.|
|Mutations Made By|| |
David McKean, Mayo Clinic
When maintaining a live colony, these mice are bred as homozygotes.
When using the Ly9- mouse strain in a publication, please cite the originating article(s) and include JAX stock #006011 in your Materials and Methods section.