Homozygous Atoh1tm2Hzo mice lack cerebellar granule neurons, cochlear and ventricular hair cells, and the pontine nuclei in the brain stem. They also display perinatal lethality.
Huda Zoghbi, Baylor College of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Atoh1 | atonal bHLH transcription factor 1 |
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mutant mice have a perinatal lethal phenotype and die shortly after birth. No gene product (protein) is detected in resting chondrocytes by immunohistochemical analysis of embryonic age 18.5 homozygotes. Beta-galactosidase X-gal staining of neural tissue from embryonic day 14.5 and newborn (postnatal day 0) aged homozygous and heterozygous mice mimicks the endogenous expression pattern. Mice homozygous for this mutation exhibit a phenotype similar to the phenotype observed in mice homozygous for the null (loss of function) targeted mutation. Homozygotes lack cerebellar granule neurons, cochlear and ventricular hair cells, and the pontine nuclei in the brain stem. This mutant mouse strain may be useful in studies of brain and inner ear development.
A targeting vector containing lacZ, neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the entire coding region. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 10 generations.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ replaces/mimics endogenous gene expression |
Allele Name | targeted mutation 2, Huda Y Zoghbi |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Atoh1LacZ; Math1/lacZ; Math1-; Math1beta-Gal; Math1lacZ |
Gene Symbol and Name | Atoh1, atonal bHLH transcription factor 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ replaces/mimics endogenous gene expression |
Strain of Origin | Not Specified |
Chromosome | 6 |
Molecular Note | A lacZ-PGK-neomycin resistance cassette replaced the coding region and placed lacZ under control of the endogenous promoter. |
Mutations Made By | Huda Zoghbi, Baylor College of Medicine |
When maintaining a live colony, these mice are bred as heterozygotes due to the homozygous perinatal lethal phenotype.
When using the Math1 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005970 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Atoh1<tm2Hzo> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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