STOCK Tg(Pomc1-cre)16Lowl/J

Stock number: 005965
Product name: Pomc-Cre
Research areas: Diabetes and Obesity Research, Neurobiology Research, Research Tools

Description

These Pomc-Cre transgenic mice express cre in in brain area neurons involved in the control of food intake, and can be bred to floxed mice to delete the floxed allele in those tissues.

Detailed description

Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre activity is demonstrable in brain area neurons involved in the control of food intake (arcuate nucleus (hypothalamus) and solitary tract nucleus (hindbrain)). When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked genome in tissues that normally express Pomc1. The mice may be useful in studies of obesity, food intake, hunger, endocrine and exocrine function, and for tissue specific gene targeting.

View cre expression characterization.

Development

A targeting vector was designed to insert a cre recombinase gene precisely into the start codon of a mouse Pomc1 gene and subsequently delete the first 30bp of the endogenous gene. This construct was microinjected into the pronuclei of fertilized one cell stage FVB/N embryos. The resulting transgenic mice (founder line 16) were bred to FVB/N for an unknown number of generations. These mice were then maintained on a mixed FVB/N, C57BL/6J, and 129 background before arrival at The Jackson Laboratory. Upon arrival, transgenic mice were bred with FVB/NJ for one generation. Transgenic mice were then maintained by breeding to wildtype siblings.

Allele

Symbol: Tg(Pomc1-cre)16Lowl
Name: transgene insertion 16, Bradford B Lowell
MGI accession ID: MGI:3640616
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Pomc1-cre)16Lowl
Marker name: transgene insertion 16, Bradford B Lowell
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: arcuate nucleus of the hypothalamus and nucleus of the solitary tract in the hindbrain
Molecular note: This transgene consists of a Pomc1 BAC such that cre is driven by Pomc1 regulatory elements. Cre is expressed in POMC neurons in the arcuate nucleus of the hypothalamus and scattered cre activity is also seen in the dentate gyrus of the hippocampus.