B6.129S1-Csf2rb2^tm1Cgb Csf2rb^tm1Clsc/J

Stock number: 005963
Product name: Bc/BIL3 KO
Research areas: Cardiovascular Research, Hematological Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Research Tools

Description

Mice homozygous for both knock-out genes exhibit eosinopenia, increased surfactant accumulation in lung tissue and pulmonary alveolar proteinosis, infection resistance, and attenuated tissue repair after injury.

Detailed description

Mice homozygous at both loci are viable and fertile with no behavioral abnormalities. Steady state hematopoiesis is normal. Bone marrow from homozygous mice fail to exhibit any cellular responses to GM-CSF, IL-5, or IL-3. Like the similar strain (Stock No. 005940; Csf2rb^tm1Cgb), double homozygous mice exhibit eosinopenia, increased surfactant accumulation in lung tissue and pulmonary alveolar proteinosis (PAP), infection resistance, and attenuated tissue repair after injury. This mutant may be useful in studies of PAP, lung physiology and disease, surfactant homeostasis and metabolism, innate immunity, cytokine signaling, myoproliferative disease, hematopoietic cell populations requiring IL-3, GM-CSF, or IL-5 for development and function, and models of ischemic, traumatic, toxic, and inflammatory injuries.

Development

A targeting vector designed to replace exons 9-13 of Csf2rb2 (β-IL3 or BIL3) with a neomycin resistance cassette was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. The ES cells determined to be heterozygous for this mutation were subsequently electroporated with another targeting vector designed to insert a hygromycin-resistance gene into exon 7 of Csf2rb (βc or Bc). Correctly targeted ES cells (containing both targeted mutations) were injected into C57 blastocysts and the resulting chimeric mice were bred with C57BL/6. Mutant mice were bred to C57BL/6 for 10 generations and then intercrossed to produce a mouse line homozygous for both mutant loci before arriving at The Jackson Laboratory.

Allele

Symbol: Csf2rb2^tm1Cgb
Name: targeted mutation 1, C Glenn Begley
MGI accession ID: MGI:3652583
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Csf2rb2
Marker name: colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)
Chromosome: 15
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: Exons 9-13 were replaced with a neomycin resistance cassette.

Allele

Symbol: Csf2rb^tm1Clsc
Name: targeted mutation 1, Clare L Scott
MGI accession ID: MGI:3653883
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Csf2rb
Marker name: colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
Chromosome: 15
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: ES cells already containing the Csf2rb2^tm1Cgb mutation were targeted a second time with a vector containing a hygromycin resistance-gene in exon 7 and a TK cassette at the upstream end of the construct. Three different ES cell lines were used to create chimeric mice.
General note: This allele was created on a chromosome already carrying the Csf2rb2^tm2Cgb allele. Mice homozygous for both alleles are referred to as betac/betaIL3 null mice.