Overview

Also Known As:Bc/BIL3 KO

Mice homozygous for both knock-out genes exhibit eosinopenia, increased surfactant accumulation in lung tissue and pulmonary alveolar proteinosis, infection resistance, and attenuated tissue repair after injury.

Donating Investigator

Lorraine Robb, The Walter and ElizaHall Institute

Genetic overview

Genetic Background	Generation

Csf2rb2^tm1Cgb

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Csf2rb2	colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)

Csf2rb^tm1Clsc

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Csf2rb	colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)

View Genetics

Research Applications

Internal/Organ Research
Research Tools
Cardiovascular Research
Hematological Research
Immunology, Inflammation and Autoimmunity Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice homozygous at both loci are viable and fertile with no behavioral abnormalities. Steady state hematopoiesis is normal. Bone marrow from homozygous mice fail to exhibit any cellular responses to GM-CSF, IL-5, or IL-3. Like the similar strain (Stock No. 005940; Csf2rb^tm1Cgb), double homozygous mice exhibit eosinopenia, increased surfactant accumulation in lung tissue and pulmonary alveolar proteinosis (PAP), infection resistance, and attenuated tissue repair after injury. This mutant may be useful in studies of PAP, lung physiology and disease, surfactant homeostasis and metabolism, innate immunity, cytokine signaling, myoproliferative disease, hematopoietic cell populations requiring IL-3, GM-CSF, or IL-5 for development and function, and models of ischemic, traumatic, toxic, and inflammatory injuries.

Development

A targeting vector designed to replace exons 9-13 of Csf2rb2 (β-IL3 or BIL3) with a neomycin resistance cassette was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. The ES cells determined to be heterozygous for this mutation were subsequently electroporated with another targeting vector designed to insert a hygromycin-resistance gene into exon 7 of Csf2rb (βc or Bc). Correctly targeted ES cells (containing both targeted mutations) were injected into C57 blastocysts and the resulting chimeric mice were bred with C57BL/6. Mutant mice were bred to C57BL/6 for 10 generations and then intercrossed to produce a mouse line homozygous for both mutant loci before arriving at The Jackson Laboratory.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Nicola NA; Robb L; Metcalf D; Cary D; Drinkwater CC; Begley CG. 1996. Functional inactivation in mice of the gene for the interleukin-3 (IL-3)-specific receptor beta-chain: implications for IL-3 function and the mechanism of receptor transmodulation in hematopoietic cells. Blood 87(7):2665-74PubMed: 8639882 MGI: J:32978
Robb L; Drinkwater CC; Metcalf D; Li R; Kontgen F; Nicola NA; Begley CG. 1995. Hematopoietic and lung abnormalities in mice with a null mutation of the common beta subunit of the receptors for granulocyte-macrophage colony-stimulating factor and interleukins 3 and 5. Proc Natl Acad Sci U S A 92(21):9565-9PubMed: 7568173 MGI: J:29737
Scott CL; Robb L; Papaevangeliou B; Mansfield R; Nicola NA; Begley CG. 2000. Reassessment of interactions between hematopoietic receptors using common beta-chain and interleukin-3-specific receptor beta-chain-null cells: no evidence of functional interactions with receptors for erythropoietin, granulocyte colony-stimulating factor, or stem cell factor. Blood 96(4):1588-90PubMed: 10942411 MGI: J:109889

View All References

Genetics

Csf2rb2^tm1Cgb

Allele Symbol: Csf2rb2^tm1Cgb

Allele Name	targeted mutation 1, C Glenn Begley
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Betail3^-; BIL3^-
Gene Symbol and Name	Csf2rb2, colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)
Gene Synonym(s)
Strain of Origin	129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Chromosome	15
Molecular Note	Exons 9-13 were replaced with a neomycin resistance cassette.

Csf2rb^tm1Clsc

Allele Symbol: Csf2rb^tm1Clsc

Allele Name	targeted mutation 1, Clare L Scott
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	betac null; Csf-2^-; Csf2rb1^tm1Clsc
Gene Symbol and Name	Csf2rb, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)
Gene Synonym(s)
Strain of Origin	129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Chromosome	15
General Note	This allele was created on a chromosome already carrying the Csf2rb2^tm2Cgb allele. Mice homozygous for both alleles are referred to as betac/betaIL3 null mice.
Molecular Note	ES cells already containing the Csf2rb2^tm2Cgb mutation were targeted a second time with a vector containing a hygromycin resistance-gene in exon 7 and a TK cassette at the upstream end of the construct. Three different ES cell lines were used to create chimeric mice.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Internal/Organ Research
- Lung Defects
- Wound Healing
Research Tools
- Toxicology Research
  - free radical research
- Immunology, Inflammation and Autoimmunity Research
  - Macrophage Deficiency
Cardiovascular Research
- Ischemia studies
- Vascular Defects
  - defective macrophage function
Hematological Research
- Hematopoietic Defects
- Immunological Defects
Immunology, Inflammation and Autoimmunity Research
- Growth Factors/Receptors/Cytokines

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Csf2rb^tm1Clsc/Csf2rb^tm1Clsc Csf2rb2^tm1Cgb/Csf2rb2^tm1Cgb
involves: 129S1/Sv

hematopoietic system phenotype

abnormal bone marrow cell number
- bone marrow cells from mice 6-14 weeks of age do not proliferate when exposed to cytokines like Il-3 or GM-CSF but response to other cytokines is normal

Genotype: Csf2rb^tm1Clsc/Csf2rb^tm1Clsc Csf2rb2^tm1Cgb/Csf2rb2^tm1Cgb
C.129S1-Csf2rb2 Csf2rb

cellular phenotype

decreased T cell proliferation
- T cells from allergic mice proliferate less vigorously in response to allergic antigen than controls

immune system phenotype

abnormal plasmacytoid dendritic cell physiology
- plasmacytoid dendritic cells have impaired migration to the lung of both na¿ve and allergy challenged mice compared to respective controls

decreased susceptibility to type I hypersensitivity reaction
- the number of mucus-secreting cells in the lung is half that of wild-type controls after intranasal antigen challenge
- 10-fold less antigen-specific IgE is detected in the sera of mice after antigen challenge
- mice fail to increase airway resistance in response to intranasal challenge with an antigen mice were previously sensitized too

decreased T cell proliferation
- T cells from allergic mice proliferate less vigorously in response to allergic antigen than controls

lymph node hyperplasia
- peribronchial lymph nodes contain about twice as more B cells, CD4⁺ T cells, and CD8⁺ T cells than in controls
- peribronchial lymph nodes also contain more myeloid and plasmacytoid dendritic cells

abnormal T cell activation
- less peribronchial lymph node T cells are in an activated state after allergy challenge compared to controls
- less activated CD4⁺ T cells are found in the lung after allergy challenge than in controls

abnormal dendritic cell physiology
- myeloid dendritic cells in the lung fail to become activated in response to intranasal allergy challenge
- dendritic cells have impaired migration to the lung of both na¿ve and allergy challenged mice compared to respective controls

decreased interleukin-13 secretion
- peribronchial lymph node T cells isolated from allergic mice produce much less IL-13 than controls upon reencounter with allergic antigen

decreased interleukin-4 secretion
- peribronchial lymph node T cells isolated from allergic mice produce much less IL-4 than controls upon reencounter with allergic antigen

increased IgG1 level
- sera levels of antigen-specific IgG1 are elevated relative to controls in response to challenge with an allergic antigen

impaired eosinophil recruitment
- eosinophil numbers in the peripheral blood, airway lumen, and lung tissue remained at baseline levels for the entire observational period of 2 wk after the final antigen challenge
- eosinophils fail to migrate to the blood, lung or bronchoalveolar fluid after intranasal challenge with an antigen mice were previously sensitized to

abnormal interleukin secretion

abnormal T cell physiology
- less CD4⁺ and CD8⁺ T cells are recruited to the lung after allergy challenge than controls

decreased interleukin-5 secretion
- peribronchial lymph node T cells isolated from allergic mice produce much less IL-5 than controls upon reencounter with allergic antigen

respiratory system phenotype

decreased airway responsiveness
- mice fail to increase airway resistance in response to intranasal antigen challenge

hematopoietic system phenotype

abnormal T cell physiology
- less CD4⁺ and CD8⁺ T cells are recruited to the lung after allergy challenge than controls

abnormal T cell activation
- less activated CD4⁺ T cells are found in the lung after allergy challenge than in controls
- less peribronchial lymph node T cells are in an activated state after allergy challenge compared to controls

increased IgG1 level
- sera levels of antigen-specific IgG1 are elevated relative to controls in response to challenge with an allergic antigen

decreased T cell proliferation
- T cells from allergic mice proliferate less vigorously in response to allergic antigen than controls

impaired eosinophil recruitment
- eosinophil numbers in the peripheral blood, airway lumen, and lung tissue remained at baseline levels for the entire observational period of 2 wk after the final antigen challenge
- eosinophils fail to migrate to the blood, lung or bronchoalveolar fluid after intranasal challenge with an antigen mice were previously sensitized to

References

Nicola NA; Robb L; Metcalf D; Cary D; Drinkwater CC; Begley CG. 1996. Functional inactivation in mice of the gene for the interleukin-3 (IL-3)-specific receptor beta-chain: implications for IL-3 function and the mechanism of receptor transmodulation in hematopoietic cells. Blood 87(7):2665-74PubMed: 8639882 MGI: J:32978
Robb L; Drinkwater CC; Metcalf D; Li R; Kontgen F; Nicola NA; Begley CG. 1995. Hematopoietic and lung abnormalities in mice with a null mutation of the common beta subunit of the receptors for granulocyte-macrophage colony-stimulating factor and interleukins 3 and 5. Proc Natl Acad Sci U S A 92(21):9565-9PubMed: 7568173 MGI: J:29737
Scott CL; Robb L; Papaevangeliou B; Mansfield R; Nicola NA; Begley CG. 2000. Reassessment of interactions between hematopoietic receptors using common beta-chain and interleukin-3-specific receptor beta-chain-null cells: no evidence of functional interactions with receptors for erythropoietin, granulocyte colony-stimulating factor, or stem cell factor. Blood 96(4):1588-90PubMed: 10942411 MGI: J:109889

Additional - Csf2rb^tm1Clsc related

Additional - Csf2rb2^tm1Cgb related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Separated PCR:Csf2rb2
- Standard PCR:Csf2rbalternate2
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice are bred homozygous at both loci.
- Additional Breeding and Husbandry Support
Citation
When using the Bc/BIL3 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005963 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Csf2rb2<tm1Cgb>, Homozygous for Csf2rb<tm1Clsc>

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Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Bc/BIL3 KO

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Csf2rb2tm1Cgb

Allele Symbol: Csf2rb2tm1Cgb

Csf2rbtm1Clsc

Allele Symbol: Csf2rbtm1Clsc

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Csf2rbtm1Clsc/Csf2rbtm1Clsc Csf2rb2tm1Cgb/Csf2rb2tm1Cgbinvolves: 129S1/Sv

hematopoietic system phenotype

Genotype: Csf2rbtm1Clsc/Csf2rbtm1Clsc Csf2rb2tm1Cgb/Csf2rb2tm1CgbC.129S1-Csf2rb2 Csf2rb

cellular phenotype

immune system phenotype

respiratory system phenotype

hematopoietic system phenotype

References

Additional - Csf2rbtm1Clsc related

Additional - Csf2rb2tm1Cgb related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Csf2rb2^tm1Cgb

Allele Symbol: Csf2rb2^tm1Cgb

Csf2rb^tm1Clsc

Allele Symbol: Csf2rb^tm1Clsc

Genotype: Csf2rb^tm1Clsc/Csf2rb^tm1Clsc Csf2rb2^tm1Cgb/Csf2rb2^tm1Cgb
involves: 129S1/Sv

Genotype: Csf2rb^tm1Clsc/Csf2rb^tm1Clsc Csf2rb2^tm1Cgb/Csf2rb2^tm1Cgb
C.129S1-Csf2rb2 Csf2rb

Additional - Csf2rb^tm1Clsc related

Additional - Csf2rb2^tm1Cgb related