Mice homozygous for both knock-out genes exhibit eosinopenia, increased surfactant accumulation in lung tissue and pulmonary alveolar proteinosis, infection resistance, and attenuated tissue repair after injury.
Lorraine Robb, The Walter and ElizaHall Institute
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Csf2rb2 | colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage) |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Csf2rb | colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) |
Mice homozygous at both loci are viable and fertile with no behavioral abnormalities. Steady state hematopoiesis is normal. Bone marrow from homozygous mice fail to exhibit any cellular responses to GM-CSF, IL-5, or IL-3. Like the similar strain (Stock No. 005940; Csf2rbtm1Cgb), double homozygous mice exhibit eosinopenia, increased surfactant accumulation in lung tissue and pulmonary alveolar proteinosis (PAP), infection resistance, and attenuated tissue repair after injury. This mutant may be useful in studies of PAP, lung physiology and disease, surfactant homeostasis and metabolism, innate immunity, cytokine signaling, myoproliferative disease, hematopoietic cell populations requiring IL-3, GM-CSF, or IL-5 for development and function, and models of ischemic, traumatic, toxic, and inflammatory injuries.
A targeting vector designed to replace exons 9-13 of Csf2rb2 (β-IL3 or BIL3) with a neomycin resistance cassette was electroporated into 129S1/Sv-derived W9.5 embryonic stem (ES) cells. The ES cells determined to be heterozygous for this mutation were subsequently electroporated with another targeting vector designed to insert a hygromycin-resistance gene into exon 7 of Csf2rb (βc or Bc). Correctly targeted ES cells (containing both targeted mutations) were injected into C57 blastocysts and the resulting chimeric mice were bred with C57BL/6. Mutant mice were bred to C57BL/6 for 10 generations and then intercrossed to produce a mouse line homozygous for both mutant loci before arriving at The Jackson Laboratory.
Allele Name | targeted mutation 1, C Glenn Begley |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Betail3-; BIL3- |
Gene Symbol and Name | Csf2rb2, colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage) |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 15 |
Molecular Note | Exons 9-13 were replaced with a neomycin resistance cassette. |
Allele Name | targeted mutation 1, Clare L Scott |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | betac null; Csf-2-; Csf2rb1tm1Clsc |
Gene Symbol and Name | Csf2rb, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 15 |
General Note | This allele was created on a chromosome already carrying the Csf2rb2tm2Cgb allele. Mice homozygous for both alleles are referred to as betac/betaIL3 null mice. |
Molecular Note | ES cells already containing the Csf2rb2tm2Cgb mutation were targeted a second time with a vector containing a hygromycin resistance-gene in exon 7 and a TK cassette at the upstream end of the construct. Three different ES cell lines were used to create chimeric mice. |
When maintaining a live colony, these mice are bred homozygous at both loci.
When using the Bc/BIL3 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005963 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Csf2rb2<tm1Cgb>, Homozygous for Csf2rb<tm1Clsc> |
Frozen Mouse Embryo | B6.129S1-Csf2rb2<tm1Cgb> Csf2rb<tm1Clsc>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Csf2rb2<tm1Cgb> Csf2rb<tm1Clsc>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Csf2rb2<tm1Cgb> Csf2rb<tm1Clsc>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Csf2rb2<tm1Cgb> Csf2rb<tm1Clsc>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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