This Cd44 knockout strain is useful in studies of embryogenesis and lymphocyte recirculation.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Cd44 | CD44 antigen |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected. Although lymphocyte development appears unremarkable, irregularities are observed in lymphocyte trafficking. Tail-injected lymphocytes derived from null animals exhibit an impaired ability to traffic to peripheral lymph nodes, and to a much greater degree, the thymus. Transcription and translation of the targeted allele subsequently lead to the synthesis of the lacZ protein under control of the 5' regulatory elements of the endogenous locus in all cells and tissues normally expressing one or several of the CD44 isoforms.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a neomycin resistance/lacZ cassette was used to disrupt exon1 and a portion of intron 1 resulting in total loss of transcription. Endogenous regulatory elements direct the transcription of the inserted neo/lacZ cassette. The construct was electroporated into 129-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6. After arrival at The Jackson Laboratory (as Stock No. 003899), SNP analysis suggested that the strain had at least one other unidentified strain in its genetic background. These mixed background were mice were then backcrossed to NOD/LtJ (Stock No. 001976) inbred mice for at least 5 generations to generate this congenic strain (Stock No. 005878).
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ expression is detected in all cells and tissues that normally express one or several CD44 isoforms. |
Allele Name | targeted mutation 1, Frank Hilberg |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | CD44- |
Gene Symbol and Name | Cd44, CD44 antigen |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ expression is detected in all cells and tissues that normally express one or several CD44 isoforms. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 2 |
Molecular Note | A promoterless lacZ gene followed by a neomycin selection cassette was fused in frame directly following the translation start codon. Northern blot analysis on RNA derived from E13.5 homozygous embryos demonstrated that no wild-type transcript was detectable. Flow cytometry analysis on cells derived from thymus, spleen and lymph nodes of homozygous mice confirmed that no stable encoded protein was expressed on the cell surface. |
Mutations Made By | Frank Hilberg, Boehringer Ingelheim R&D Vienna |
When maintaining a live colony, these mice are bred as homozygotes.
When using the CD44 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005878 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cd44<tm1Hbg> |
Frozen Mouse Embryo | NOD.129(Cg)-Cd44<tm1Hbg>/J | $2595.00 |
Frozen Mouse Embryo | NOD.129(Cg)-Cd44<tm1Hbg>/J | $2595.00 |
Frozen Mouse Embryo | NOD.129(Cg)-Cd44<tm1Hbg>/J | $3373.50 |
Frozen Mouse Embryo | NOD.129(Cg)-Cd44<tm1Hbg>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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