C57BL/6J-Tg(ACTB-DDAH1)1Jpck/J

Stock number: 005863
Product name: hDDAH-1
Strain synonyms: hDDAH transgenic mice, DDAH transgenic mice
Research areas: Cardiovascular Research, Diabetes and Obesity Research, Internal/Organ Research, Metabolism Research

Description

These hDDAH-1 mice express a transgene containing human dimethylarginine dimethylaminohydrolase-1 (DDAH-1) and exhibit a reduction of plasma asymmetric dimethylarginine and an increase in nitric oxide synthase (NOS) activity.

Detailed description

Hemizygous mice are viable and fertile with no anatomic abnormalities. Transgene expression is observed in aorta, heart, and brain. Transgenic dimethylarginine dimethylaminohydrolase (DDAH) activity is reflected in a reduction of plasma asymmetric dimethylarginine (ADMA). Nitric oxide synthase (NOS) activity is significantly increased in transgenic skeletal and cardiac muscle. Transgenic mice have lower blood pressure and higher heart rate. Transgenic mice may be useful in studies of atherosclerosis, hypertension, hyperlipidemia, hypercholesterolemia, hyperhomocystinemia, diabetes mellitus, stroke, renal failure, preeclampsia, and other conditions associated with vascular pathophysiology and/or cardiovascular disease.

Development

A human DDAH-I cDNA and SV40 derived from a modified human agouti expression vector were placed under control of the human B-actin promoter. This construct was injected into fertilized C57BL/6J eggs. Embryos were transferred into the oviducts of pseudopregnant B6CBAF1 foster mothers. Transgenic males were bred to C57BL/6J approximately 8-10 generations before arrival at The Jackson Laboratory.

Allele

Symbol: Tg(ACTB-DDAH1)1Jpck
Name: transgene insertion 1, John P Cooke
MGI accession ID: MGI:3696379
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Synonyms: DDAH; DDAH1 TG
Marker symbol: Tg(ACTB-DDAH1)1Jpck
Marker name: transgene insertion 1, John P Cooke
Marker synonyms: DDAH
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: DDAH1,dimethylarginine dimethylaminohydrolase 1,human
Molecular note: A human DDAH-I cDNA and SV40 derived from a modified human agouti expression vector were placed under control of the human B-actin promoter.