B6.129S6-Esam1^tm1Tq/J

Stock number: 005862
Research areas: Cancer Research, Cell Biology Research, Developmental Biology Research

Description

This Esam1 knockout strain is useful in studies of cellular adhesion, vascular integrity, and angiogenesis.

Detailed description

Homozygous mice are viable with no histologically observable abnormalities. Homozygotes do not express the targeted gene in lung, kidney, heart, aorta, liver, skin, and eye. Homozygous mice have a 15% decrease in body weight compared to wild type. Endothelial cells derived from wild type mice form tube-shaped primitive blood vessels structures. Cultured aortic endothelial cells from homozygote mutants display an attenuated ability to form tubes, suggesting diminished angiogenic potential. Cultured cells from mutant mice also exhibit a decreased migratory response to basic fibroblast growth factor in vitro. Following injection with melanoma and Lewis lung carcinoma cells, homozygous mice show retarded tumor growth correlated with decreased angiogenesis. This mouse may be useful in studies of cellular adhesion, vascular integrity, and angiogenesis, as well as pathologic blood vessel assembly.

Development

A targeting vector containing a beta-galactosidase and neomycin resistance gene was used to replace exon 1 of the endogenous gene. The construct was electroporated into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Three resulting chimeric male animals were bred to C57BL/6J females. Mutant mice were mated to C57BL/6J for more than 5 generations before arrival at The Jackson Laboratory.

Allele

Symbol: Esam^tm1Tq
Name: targeted mutation 1, Thomas Quertermous
MGI accession ID: MGI:2675994
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Esam
Marker name: endothelial cell-specific adhesion molecule
Chromosome: 9
Strain of origin: 129S6/SvEvTac
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: A 3' portion of exon 1, beginning 20 bp upstream of the start codon, was replaced with a cassette containing beta-galactosidase and neomycin resistance genes. Normal transcript was undetected in various tissues including the kidney, heart, and lung of homozygous mutant mice by Northern blot analysis.