This Esam1 knockout strain is useful in studies of cellular adhesion, vascular integrity, and angiogenesis.
Thomas Quertermous, Stanford University School of Medicine
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Esam | endothelial cell-specific adhesion molecule |
Homozygous mice are viable with no histologically observable abnormalities. Homozygotes do not express the targeted gene in lung, kidney, heart, aorta, liver, skin, and eye. Homozygous mice have a 15% decrease in body weight compared to wild type. Endothelial cells derived from wild type mice form tube-shaped primitive blood vessels structures. Cultured aortic endothelial cells from homozygote mutants display an attenuated ability to form tubes, suggesting diminished angiogenic potential. Cultured cells from mutant mice also exhibit a decreased migratory response to basic fibroblast growth factor in vitro. Following injection with melanoma and Lewis lung carcinoma cells, homozygous mice show retarded tumor growth correlated with decreased angiogenesis. This mouse may be useful in studies of cellular adhesion, vascular integrity, and angiogenesis, as well as pathologic blood vessel assembly.
A targeting vector containing a beta-galactosidase and neomycin resistance gene was used to replace exon 1 of the endogenous gene. The construct was electroporated into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Three resulting chimeric male animals were bred to C57BL/6J females. Mutant mice were mated to C57BL/6J for more than 5 generations before arrival at The Jackson Laboratory.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, Thomas Quertermous |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | ESAM-; Esam1- |
Gene Symbol and Name | Esam, endothelial cell-specific adhesion molecule |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 9 |
Molecular Note | A 3' portion of exon 1, beginning 20 bp upstream of the start codon, was replaced with a cassette containing beta-galactosidase and neomycin resistance genes. Normal transcript was undetected in various tissues including the kidney, heart, and lung of homozygous mutant mice by Northern blot analysis. |
Mutations Made By | Thomas Quertermous, Stanford University School of Medicine |
When maintaining a live colony, these mice are bred as heterozygotes. Homozygous mice have reported breeding problems of an unspecified nature.
When using the B6.129S6-Esam1tm1Tq/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005862 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Esam1<tm1Tq> |
Frozen Mouse Embryo | B6.129S6-Esam1<tm1Tq>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Esam1<tm1Tq>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Esam1<tm1Tq>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Esam1<tm1Tq>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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