B6.129P2-Galr1^tm1Dgen/J

Stock number: 005809
Research areas: Neurobiology Research, Research Tools

Detailed description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Development

The construct insert is reported to be "IRES-lacZ-Neo555G." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 6 generations to C57BL/6 mice.

Allele

Symbol: Galr1^tm1Dgen
Name: targeted mutation 1, Deltagen
MGI accession ID: MGI:3604584
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Galr1
Marker name: galanin receptor 1
Chromosome: 18
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ expression is not detected in any tissue or organs examined.
Molecular note: A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in the pancreas and lower levels in brain, brainstem, spinal cord, pancreas pituitary gland, adrenal gland, salivary gland, tongue, stomach, small intestine, large intestine, cecum, testis and epididymis. Beta-galactosidase expression from this allele is not detectable in any tissues or organs analyzed.