These Axl KO mice may be useful in studies of innate immunity, autoimmunity, germ cell development and apoptosis. Axl is highly expressed by key cellular targets of ZIKA virus (ZIKV), and is proposed to promote viral entry in certain cell types (including non-Axl-expressing cells). Axl inhibition represents a potential approach for antiviral therapies.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Axl | AXL receptor tyrosine kinase |
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.
Briefly, a bacterial beta-galactosidase gene (lacZ) was inserted into the Axl locus such that the endogenous gene promoter drives lacZ expression. RNA transcripts are detectable in all tissues analyzed. lacZ expression is detectable in all tissues or organs analyzed. There were no significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wildtype control mice.
In combination with mutations in other receptor tyrosine kinases, this strain may be useful in studies of innate immunity, autoimmunity, germ cell development and apoptosis.
The TAM receptors (Tyro3, Axl, and Mertk) are a family of receptor tyrosine kinases whose ligands (Gas6 and Protein S) bind to phosphatidylserine on the surface of apoptotic cells and enveloped viruses. Axl is highly expressed by key cellular targets of ZIKA virus (ZIKV) - including human radial glial cells, astrocytes, endothelial cells and microglia in developing human cortex, and by progenitor cells in developing retina (Nowakowski et al. 2016 Cell Stem Cell 18:591), as well as in subtypes of trophoblasts (Tabata et al. 2016 Cell Host Microb. 20:155). Research in 2017 identifies a dual role of Axl during ZIKV infection of human glial cells (astrocytes and microglia) - promoting viral entry and modulating innate immune responses - and suggests Axl inhibition may represent a potential target for antiviral therapies (Meertens et al. 2017 Cell Rep. 18:324).
This Axl KO allele has a Lac0-SA-IRES-lacZ-Neo555G/Kan cassette replacing exons 1-11 of the AXL receptor tyrosine kinase (Axl) gene. Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least six generations to C57BL/6 mice.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | lacZ expression is detected in: brain, spinal cord, sciatic nerve, eyes, Harderian glands, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, gallbladder, pancreas, kidney, urinary bladder, trachea, larynx, esophagus, thyroid gland, parathyroid gland, pituitary gland, adrenal glands, salivary glands, tongue, skeletal muscle, skin, male and female reproductive systems. With strong expression detected in smooth muscle cells, blood vessels, capsules and connective tissue. |
Allele Name | targeted mutation 1, Deltagen |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Axl, AXL receptor tyrosine kinase |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | lacZ expression is detected in: brain, spinal cord, sciatic nerve, eyes, Harderian glands, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, gallbladder, pancreas, kidney, urinary bladder, trachea, larynx, esophagus, thyroid gland, parathyroid gland, pituitary gland, adrenal glands, salivary glands, tongue, skeletal muscle, skin, male and female reproductive systems. With strong expression detected in smooth muscle cells, blood vessels, capsules and connective tissue. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 7 |
Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RNA transcripts are detectable in all tissues analyzed. LacZ (beta-galactosidase) expression is detectable in all tissues or organs analyzed. |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Alternatively, homozygous mice may be bred together.
When using the Axl KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #005777 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Axl<tm1Dgen> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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