Overview

This strain may be useful in studies of innate immunity, autoimmunity, germ cell development and apoptosis. Axl is highly expressed by key cellular targets of ZIKA virus (ZIKV), and is proposed to promote viral entry in certain cell types (including non-Axl-expressing cells). Axl inhibition represents a potential approach for antiviral therapies.

Genetic overview

Genetic Background	Generation

Axl^tm1Dgen

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Axl	AXL receptor tyrosine kinase

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Cell Biology Research
Research Tools
Virology Research
Apoptosis Research
Reproductive Biology Research

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Briefly, a bacterial beta-galactosidase gene (lacZ) was inserted into the Axl locus such that the endogenous gene promoter drives lacZ expression. RNA transcripts are detectable in all tissues analyzed. lacZ expression is detectable in all tissues or organs analyzed. There were no significant differences detected in the homozygous mutant mice when compared with age- and gender-matched wildtype control mice.

In combination with mutations in other receptor tyrosine kinases, this strain may be useful in studies of innate immunity, autoimmunity, germ cell development and apoptosis.

The TAM receptors (Tyro3, Axl, and Mertk) are a family of receptor tyrosine kinases whose ligands (Gas6 and Protein S) bind to phosphatidylserine on the surface of apoptotic cells and enveloped viruses. Axl is highly expressed by key cellular targets of ZIKA virus (ZIKV) - including human radial glial cells, astrocytes, endothelial cells and microglia in developing human cortex, and by progenitor cells in developing retina (Nowakowski et al. 2016 Cell Stem Cell 18:591), as well as in subtypes of trophoblasts (Tabata et al. 2016 Cell Host Microb. 20:155). Research in 2017 identifies a dual role of Axl during ZIKV infection of human glial cells (astrocytes and microglia) - promoting viral entry and modulating innate immune responses - and suggests Axl inhibition may represent a potential target for antiviral therapies (Meertens et al. 2017 Cell Rep. 18:324).

Development

The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least six generations to C57BL/6 mice.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression is detected in: brain, spinal cord, sciatic nerve, eyes, Harderian glands, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, gallbladder, pancreas, kidney, urinary bladder, trachea, larynx, esophagus, thyroid gland, parathyroid gland, pituitary gland, adrenal glands, salivary glands, tongue, skeletal muscle, skin, male and female reproductive systems. With strong expression detected in smooth muscle cells, blood vessels, capsules and connective tissue.

Expressed Gene

lacZ, beta-galactosidase, E. coli

Site of Expression

lacZ expression is detected in: brain, spinal cord, sciatic nerve, eyes, Harderian glands, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, gallbladder, pancreas, kidney, urinary bladder, trachea, larynx, esophagus, thyroid gland, parathyroid gland, pituitary gland, adrenal glands, salivary glands, tongue, skeletal muscle, skin, male and female reproductive systems. With strong expression detected in smooth muscle cells, blood vessels, capsules and connective tissue.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Genetics

Axl^tm1Dgen

Allele Symbol: Axl^tm1Dgen

Allele Name	targeted mutation 1, Deltagen
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	Axl^tm1Dgen; targeted mutation 1, Deltagen
Gene Symbol and Name	Axl, AXL receptor tyrosine kinase
Gene Synonym(s)	UFO; expressed sequence AI323647; Tyro7; Ufo; JTK11; AI323647; ufo oncogene homolog; ARK; Ark; Ufo
Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression	lacZ expression is detected in: brain, spinal cord, sciatic nerve, eyes, Harderian glands, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, gallbladder, pancreas, kidney, urinary bladder, trachea, larynx, esophagus, thyroid gland, parathyroid gland, pituitary gland, adrenal glands, salivary glands, tongue, skeletal muscle, skin, male and female reproductive systems. With strong expression detected in smooth muscle cells, blood vessels, capsules and connective tissue.
Strain of Origin	129P2/OlaHsd
Chromosome	7
Molecular Note	A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RNA transcripts are detectable in all tissues analyzed. LacZ (beta-galactosidase) expression is detectable in all tissues or organs analyzed.

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Vaccine Development
- Intracellular Signaling Molecules
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - genes regulating susceptibility to infectious disease and endotoxin
- Autoimmunity
Cell Biology Research
- Signal Transduction
Research Tools
- Cell Biology Research
- Immunology, Inflammation and Autoimmunity Research
  - genes regulating susceptibility to infectious disease and endotoxin
- Infectious Disease
- Apoptosis Research
- Reproductive Biology Research
- lacZ Expression
Virology Research
Apoptosis Research
- Endogenous Regulators
  - Autoimmune Lymphroliferatve syndrome
Reproductive Biology Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Axl^tm1Dgen/Axl^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

normal phenotype

no abnormal phenotype detected

References

Additional - Axl^tm1Dgen related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Axl^tm1Dgen
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Alternatively, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the B6.129P2-Axl^tm1Dgen/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #005777 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or Wild-type for Axl<tm1Dgen>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Questions about Terms of Use

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Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Axltm1Dgen

Allele Symbol: Axltm1Dgen

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Axltm1Dgen/Axltm1Dgeninvolves: 129P2/OlaHsd * C57BL/6

normal phenotype

References

Additional - Axltm1Dgen related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Axl^tm1Dgen

Allele Symbol: Axl^tm1Dgen

Genotype: Axl^tm1Dgen/Axl^tm1Dgen
involves: 129P2/OlaHsd * C57BL/6

Additional - Axl^tm1Dgen related