B6.129P2-Adamts5^tm1Dgen/J

Stock number: 005771
Research areas: Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Detailed description

This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.

Development

The construct insert is reported to be "LacO-SA-IRES-lacZ-Neo555G/Kan." Upon arrival, these mice had been backcrossed four generations to C57BL/6. During the importation process at The Jackson Laboratory, at least one additional backcross to C57BL/6J mice was performed. The resulting mice are backcrossed a total of at least five generations.

Allele

Symbol: Adamts5^tm1Dgen
Name: targeted mutation 1, Deltagen
MGI accession ID: MGI:3604488
Generation method: Targeted
Attributes: Reporter; Null/Knockout; Modified regulatory region
Marker symbol: Adamts5
Marker name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Chromosome: 16
Strain of origin: 129P2/OlaHsd
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ expression is detected in: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, trachea, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix.
Molecular note: An IRES-lacZ gene was inserted into and replaced 134 nucleotides of exon 2. The deleted region includes sequences encoding the catalytic active site as well as most of the putative muscle regulatory factor-binding enhancer. The highest levels of RNA transcripts are detectable in pancreas, adrenal gland and white fat. Moderate levels of RNA transcripts are detectable in: olfactory bulb, heart, lung, thymus, skin, gallbladder, testis, epididymis and coagulating gland. Lower levels of RNA transcripts are also detectable in: whole brain, cortex, subcortical region, cerebellum, brainstem, spinal cord, eye, Harderian glands, liver, kidney, spleen, lymph nodes, bone marrow, urinary bladder, pituitary gland, salivary gland, skeletal muscle, tongue, stomach, small intestine, large intestine, cecum, seminal vesicle, prostate gland and uterus. LacZ expression was detected in all organs examined.