STOCK Ppif^tm1Mmos/J

Stock number: 005737
Product name: CypD floxed
Research areas: Cell Biology Research, Metabolism Research, Research Tools

Description

These floxed mutant mice possess loxP sites flanking exons 3-5 of the Ppif gene. This strain may be useful for generating conditional mutations in applications examining the consequences of disrupting Ppif-dependent pathways.

Detailed description

In this strain loxP sites flank exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination results in the deletion of the loxP-flanked region. This strain represents an effective tool for generating tissue-specific targeted mutants useful in studies examining the consequences of disrupting Ppif-dependent pathways.

Development

A targeting vector containing a loxP-flanked neomycin resistance gene cassette preceding exons 3-5 and a third loxP site downstream of exon 5 was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pMC-Cre gene to delete the neo gene (resulting in loxP-flanked exons 3-5 [CypD^flox]) and then injected into C57BL/6 blastocysts. Chimeric mice were crossed to C57BL/6. Heterozygotes were intercrossed to generate homozygotes (CypD^f/f) and these mice were maintained on a "mixed" background by Dr. Stanley Korsmeyer at the Dana-Farber Cancer Institute. In 2005, homozygous male mice on a mixed background were sent to Dr. Michael Forte at the Vollum Institute, Oregon Health and Sciences University. The mice were bred to C57BL/6J for one generation and then made homozygous prior to arrival at The Jackson Laboratory.

Allele

Symbol: Ppif^tm1Mmos
Name: targeted mutation 1, Michael A Moskowitz
MGI accession ID: MGI:3624425
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: CypD^flox
Marker symbol: Ppif
Marker name: peptidylprolyl isomerase F (cyclophilin F)
Marker synonyms: CYP3; CypD; Cyp-D; CyP-F; CyP-M; mitochondrial Cyclophilin D; PPIase
Chromosome: 14
Strain of origin: 129X1/SvJ
Molecular note: LoxP sites flank exons 3-5.