These floxed mutant mice possess loxP sites flanking exons 3-5 of the Ppif gene. This strain may be useful for generating conditional mutations in applications examining the consequences of disrupting Ppif-dependent pathways.
Dr. Stanley J. Korsmeyer, Dana-Farber Cancer Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ppif | peptidylprolyl isomerase F (cyclophilin F) |
In this strain loxP sites flank exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination results in the deletion of the loxP-flanked region. This strain represents an effective tool for generating tissue-specific targeted mutants useful in studies examining the consequences of disrupting Ppif-dependent pathways.
A targeting vector containing a loxP-flanked neomycin resistance gene cassette preceding exons 3-5 and a third loxP site downstream of exon 5 was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pMC-Cre gene to delete the neo gene (resulting in loxP-flanked exons 3-5 [CypDflox]) and then injected into C57BL/6 blastocysts. Chimeric mice were crossed to C57BL/6. Heterozygotes were intercrossed to generate homozygotes (CypDf/f) and these mice were maintained on a "mixed" background by Dr. Stanley Korsmeyer at the Dana-Farber Cancer Institute. In 2005, homozygous male mice on a mixed background were sent to Dr. Michael Forte at the Vollum Institute, Oregon Health and Sciences University. The mice were bred to C57BL/6J for one generation and then made homozygous prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, Michael A Moskowitz |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | CypDflox |
Gene Symbol and Name | Ppif, peptidylprolyl isomerase F (cyclophilin F) |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 14 |
Molecular Note | LoxP sites flank exons 3-5. |
Mutations Made By | Dr. Stanley Korsmeyer, Dana-Farber Cancer Institute |
When maintaining a live colony, these mice are maintained as homozygotes.
When using the CypD floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #005737 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ppif<tm1Mmos> |
Frozen Mouse Embryo | STOCK Ppif<tm1Mmos>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Ppif<tm1Mmos>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Ppif<tm1Mmos>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | STOCK Ppif<tm1Mmos>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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