These floxed mutant mice possess loxP sites flanking exons 3-5 of the Ppif gene. This strain may be useful for generating conditional mutations in applications examining the consequences of disrupting Ppif-dependent pathways.
Dr. Stanley J. Korsmeyer, Dana-Farber Cancer Institute
In this strain loxP sites flank exons 3-5 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cre-mediated recombination results in the deletion of the loxP-flanked region. This strain represents an effective tool for generating tissue-specific targeted mutants useful in studies examining the consequences of disrupting Ppif-dependent pathways.
A targeting vector containing a loxP-flanked neomycin resistance gene cassette preceding exons 3-5 and a third loxP site downstream of exon 5 was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a pMC-Cre gene to delete the neo gene (resulting in loxP-flanked exons 3-5 [CypDflox]) and then injected into C57BL/6 blastocysts. Chimeric mice were crossed to C57BL/6. Heterozygotes were intercrossed to generate homozygotes (CypDf/f) and these mice were maintained on a "mixed" background by Dr. Stanley Korsmeyer at the Dana-Farber Cancer Institute. In 2005, homozygous male mice on a mixed background were sent to Dr. Michael Forte at the Vollum Institute, Oregon Health and Sciences University. The mice were bred to C57BL/6J for one generation and then made homozygous prior to arrival at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Michael A Moskowitz|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Ppif, peptidylprolyl isomerase F (cyclophilin F)|
|Strain of Origin||129X1/SvJ|
|Molecular Note||LoxP sites flank exons 3-5.|
|Mutations Made By|| |
Dr. Stanley Korsmeyer, Dana-Farber Cancer Institute
When maintaining a live colony, these mice are maintained as homozygotes.
When using the CypD floxed mouse strain in a publication, please cite the originating article(s) and include JAX stock #005737 in your Materials and Methods section.