This strain expresses Cre recombinase in thymocytes and is active in T-cells during development.
Alexander Chervonsky, The University of Chicago
Genetic Background | Generation |
---|---|
001289 NOD/ShiLt |
|
Allele Type |
---|
Transgenic (Recombinase-expressing) |
This strain expresses Cre recombinase in thymocytes and is active in T-cells during development.
The transgenic allele containing a Cre recombinase gene under the control of the Lck promoter from Stock No. 003802 (B6.Cg-Tg(Lck-cre)548Jxm/J) was transferred to the NOD/Lt background through more than 10 generations of backcrossing. In 2005, the T1DR received hemizygous mice from this congenic NOD transgenic strain at generation N12.
In Goodwin et al. 2019 Genome Res 29:494, it was discovered that Stock No. 003802 has more than 20 transgene copies integrated on chromosome 11 [41,490,714]. This study reports that no gene/locus was disrupted.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | thymocytes |
Allele Name | transgene insertion 548, Jamey Marth |
---|---|
Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Lck-cre; pLck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm |
Gene Symbol and Name | Tg(Lck-cre)548Jxm, transgene insertion 548, Jamey Marth |
Gene Synonym(s) | |
Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | thymocytes |
Strain of Origin | (C57BL/6 x CBA)F2 |
Chromosome | 11 |
General Note | Homozygous transgenic mice are viable and have no major defects. |
Molecular Note | This transgene expresses Cre recombinase with a human growth hormone polyadenylation signal under the control of the mouse lck promoter, active in the T cell lineage. Transgene insertion occurred on Chr11, causing a 1 bp duplication. |
Mutations Made By | Jamey Marth, Burnham Inst at Univ Calif Santa Barbara |
When using the NOD.Lck-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #005732 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Lck-cre)548Jxm |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.