NOD.Cg-Tg(Lck-cre)548Jxm/AchJ

Stock number: 005732
Product name: NOD.Lck-cre
Research areas: Diabetes and Obesity Research, Research Tools

Detailed description

This strain expresses Cre recombinase in thymocytes and is active in T-cells during development.

Development

The transgenic allele containing a Cre recombinase gene under the control of the Lck promoter from Stock No. 003802 (B6.Cg-Tg(Lck-cre)548Jxm/J) was transferred to the NOD/Lt background through more than 10 generations of backcrossing. In 2005, the T1DR received hemizygous mice from this congenic NOD transgenic strain at generation N12.

In Goodwin et al. 2019 Genome Res 29:494, it was discovered that Stock No. 003802 has more than 20 transgene copies integrated on chromosome 11 [41,490,714]. This study reports that no gene/locus was disrupted.

Allele

Symbol: Tg(Lck-cre)548Jxm
Name: transgene insertion 548, Jamey Marth
MGI accession ID: MGI:2176199
Generation method: Transgenic
Attributes: Recombinase-expressing
Synonyms: Lck-cre; pLck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm
Marker symbol: Tg(Lck-cre)548Jxm
Marker name: transgene insertion 548, Jamey Marth
Marker synonyms: Lck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm
Chromosome: 11
Strain of origin: (C57BL/6 x CBA)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: thymocytes
Molecular note: This transgene expresses Cre recombinase with a human growth hormone polyadenylation signal under the control of the mouse lck promoter, active in the T cell lineage. Transgene insertion occurred on Chr11, causing a 1 bp duplication.
General note: Homozygous transgenic mice are viable and have no major defects.