These B6.H2g7RIPB7.1 mice express a transgene encoding the human CD80 T cell co-stimulatory molecule and most mice become diabetic.
Li Wen, Yale School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Not Applicable | H2 | histocompatibility-2, MHC |
Transgenic mice are characterized by pancreatic beta cells that express a rat insulin promoter (Ins2) regulated transgene encoding the human CD80 T cell co-stimulatory molecule. These mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Better than 70% of the B6.H2g7 transgenic mice become diabetic by 30 weeks of age compared the control B6.H2g7 which does not develop insulitis or diabetes. Spleens of diabetic B6.H2g7 transgenic mice used in adoptive transfer experiments transfer diabetes to NOD.scid/RIP-B7.1 and irradiated non-diabetic B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ mice, yet failed to transfer disease to NOD.scid, B6.scid, CB17.scid, or irradiated B6/RIP-B7.1.
B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ provides a tool for studying mechanisms of loss of tolerance in potentially diabetogenic CD8 T-cells.
This transgene is available on the following additional backgrounds: C.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ (Stock 005713) and NOR.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ (Stock 005714).
A transgenic construct containing the human CD80 gene driven by the rat insulin promoter 1 (RIP) was injected fertilized eggs of a mating between C57BL/6 and CBA/Ca strains in the laboratory of Dr. Richard Flavell (Yale University). Founder animals were obtained and bred to C57BL/6 mice for more than 10 generations and B6.NOD-H2g7 for 1 generation, progeny were selected for the H2g7 allele. In 2005, The Jackson Laboratory received this B6.Cg-H2g7Tg(Ins2-CD80)3B7Flv/LwnJ at N1F9.
Expressed Gene | CD80, CD80 molecule, human |
---|---|
Site of Expression | |
Site of Expression |
Allele Name | transgene insertion 3B7, Richard Flavell |
---|---|
Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | RIP-B7; RIP-B7.1; RIP-B7-1; RIP-CD80 |
Gene Symbol and Name | Tg(Ins2-CD80)3B7Flv, transgene insertion 3B7, Richard Flavell |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | CD80, CD80 molecule, human |
Strain of Origin | (C57BL/6 x CBA/Ca)F2 |
Chromosome | UN |
General Note | Mice carrying this transgene that also are homozygous for Prkdcscid are characterized by pancreatic beta cells that express a rat insulin II promoter regulated transgene encoding the human CD80 T cell co-stimulatory molecule. |
Molecular Note | The transgene contains a human CD80 antigen gene driven by the rat insulin II promoter (Ins2). |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
Allele Name | g7 variant |
---|---|
Allele Type | Not Applicable |
Allele Synonym(s) | H-2g7; H-2g7 |
Gene Symbol and Name | H2, histocompatibility-2, MHC |
Gene Synonym(s) | |
Strain of Origin | Not Applicable |
Chromosome | 17 |
General Note | The g7 variant has been observed in the following strains: DBR7, NON.NOD-H2g7 |
When using the B6.H2g7RIPB7.1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005715 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Ins2-CD80)3B7Flv |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.