B6.Cg-H2^g7 Tg(Ins2-CD80)3B7Flv/LwnJ

Stock number: 005715
Product name: B6.H2^g7RIPB7.1
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These B6.H2g7RIPB7.1 mice express a transgene encoding the human CD80 T cell co-stimulatory molecule and most mice become diabetic.

Detailed description

Transgenic mice are characterized by pancreatic beta cells that express a rat insulin promoter (Ins2) regulated transgene encoding the human CD80 T cell co-stimulatory molecule. These mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Better than 70% of the B6.H2^g7 transgenic mice become diabetic by 30 weeks of age compared the control B6.H2^g7 which does not develop insulitis or diabetes. Spleens of diabetic B6.H2^g7 transgenic mice used in adoptive transfer experiments transfer diabetes to NOD.scid/RIP-B7.1 and irradiated non-diabetic B6.Cg H2^g7-Tg(Ins2-CD80)3B7Flv/LwnJ mice, yet failed to transfer disease to NOD.scid, B6.scid, CB17.scid, or irradiated B6/RIP-B7.1.

B6.Cg H2^g7-Tg(Ins2-CD80)3B7Flv/LwnJ provides a tool for studying mechanisms of loss of tolerance in potentially diabetogenic CD8 T-cells.

This transgene is available on the following additional backgrounds: C.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ (Stock 005713) and NOR.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ (Stock 005714).

Development

A transgenic construct containing the human CD80 gene driven by the rat insulin promoter 1 (RIP) was injected fertilized eggs of a mating between C57BL/6 and CBA/Ca strains in the laboratory of Dr. Richard Flavell (Yale University). Founder animals were obtained and bred to C57BL/6 mice for more than 10 generations and B6.NOD-H2^g7 for 1 generation, progeny were selected for the H2^g7 allele. In 2005, The Jackson Laboratory received this B6.Cg-H2^g7Tg(Ins2-CD80)3B7Flv/LwnJ at N1F9.

Allele

Symbol: Tg(Ins2-CD80)3B7Flv
Name: transgene insertion 3B7, Richard Flavell
MGI accession ID: MGI:2682826
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Ins2-CD80)3B7Flv
Marker name: transgene insertion 3B7, Richard Flavell
Chromosome: UN
Strain of origin: (C57BL/6 x CBA/Ca)F2
Expressed genes: CD80,CD80 molecule,human
Molecular note: The transgene contains a human CD80 antigen gene driven by the rat insulin II promoter (Ins2).
General note: Mice carrying this transgene that also are homozygous for Prkdc^scid are characterized by pancreatic beta cells that express a rat insulin II promoter regulated transgene encoding the human CD80 T cell co-stimulatory molecule.

Allele

Symbol: H2^g7
Name: g7 variant
MGI accession ID: MGI:3579321
Generation method: Not Applicable
Marker symbol: H2
Marker name: histocompatibility-2, MHC
Chromosome: 17
Strain of origin: Not Applicable
General note: The g7 variant has been observed in the following strains: DBR7, NON.NOD-H2^g7