C.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ

Stock number: 005713
Product name: BALB-RIPB7.1
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These BALB-RIPB7.1 mice express a transgene encoding the human CD80 T cell co-stimulatory molecule and approximately 10% of the mice become diabetic.

Detailed description

Transgenic mice are characterized by pancreatic beta cells that express a rat insulin promoter (Ins2) regulated transgene encoding the human CD80 T cell co-stimulatory molecule. These mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Approximately 10% of the BALB transgenic mice become diabetic by 30 weeks of age compared the control BALBs which are diabetes resistant. Spleens of diabetic BALB transgenic mice used in adoptive transfer experiments do not transfer diabetes to NOD.scid/RIP-B7.1.

C.Cg-Tg(Ins2-CD80)3B7Flv/FswJ provides a tool for studying mechanisms for loss of tolerance in potentially diabetogenic CD8 T-cells.

This transgene is available on the following additional backgrounds: B6.Cg H2^g7-Tg(Ins2-CD80)3B7Flv/LwnJ (Stock 005715) and NOR.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ (Stock 005714).

Development

A transgenic construct containing the human CD80 gene driven by the rat insulin promoter 1 (Rip) was injected fertilized eggs of a mating between C57BL/6 and CBA/Ca strains in the laboratory of Dr. Richard Flavell (Yale University). Founder animals were obtained and bred to C57BL/6 mice and subsequently mated to BALB/c for 10 generations. In 2005, The Jackson Laboratory received this C.Cg-Tg(Ins2-CD80)3B7FLV/FswJ at N10F5.

Allele

Symbol: Tg(Ins2-CD80)3B7Flv
Name: transgene insertion 3B7, Richard Flavell
MGI accession ID: MGI:2682826
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Ins2-CD80)3B7Flv
Marker name: transgene insertion 3B7, Richard Flavell
Chromosome: UN
Strain of origin: (C57BL/6 x CBA/Ca)F2
Expressed genes: CD80,CD80 molecule,human
Molecular note: The transgene contains a human CD80 antigen gene driven by the rat insulin II promoter (Ins2).
General note: Mice carrying this transgene that also are homozygous for Prkdc^scid are characterized by pancreatic beta cells that express a rat insulin II promoter regulated transgene encoding the human CD80 T cell co-stimulatory molecule.