These BALB-RIPB7.1 mice express a transgene encoding the human CD80 T cell co-stimulatory molecule and approximately 10% of the mice become diabetic.
Li Wen, Yale School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Transgenic mice are characterized by pancreatic beta cells that express a rat insulin promoter (Ins2) regulated transgene encoding the human CD80 T cell co-stimulatory molecule. These mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Approximately 10% of the BALB transgenic mice become diabetic by 30 weeks of age compared the control BALBs which are diabetes resistant. Spleens of diabetic BALB transgenic mice used in adoptive transfer experiments do not transfer diabetes to NOD.scid/RIP-B7.1.
C.Cg-Tg(Ins2-CD80)3B7Flv/FswJ provides a tool for studying mechanisms for loss of tolerance in potentially diabetogenic CD8 T-cells.
This transgene is available on the following additional backgrounds: B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ (Stock 005715) and NOR.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ (Stock 005714).
A transgenic construct containing the human CD80 gene driven by the rat insulin promoter 1 (Rip) was injected fertilized eggs of a mating between C57BL/6 and CBA/Ca strains in the laboratory of Dr. Richard Flavell (Yale University). Founder animals were obtained and bred to C57BL/6 mice and subsequently mated to BALB/c for 10 generations. In 2005, The Jackson Laboratory received this C.Cg-Tg(Ins2-CD80)3B7FLV/FswJ at N10F5.
Expressed Gene | CD80, CD80 molecule, human |
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Site of Expression |
Allele Name | transgene insertion 3B7, Richard Flavell |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | RIP-B7; RIP-B7.1; RIP-B7-1; RIP-CD80 |
Gene Symbol and Name | Tg(Ins2-CD80)3B7Flv, transgene insertion 3B7, Richard Flavell |
Gene Synonym(s) | |
Promoter | Ins2, insulin 2, rat |
Expressed Gene | CD80, CD80 molecule, human |
Strain of Origin | (C57BL/6 x CBA/Ca)F2 |
Chromosome | UN |
General Note | Mice carrying this transgene that also are homozygous for Prkdcscid are characterized by pancreatic beta cells that express a rat insulin II promoter regulated transgene encoding the human CD80 T cell co-stimulatory molecule. |
Molecular Note | The transgene contains a human CD80 antigen gene driven by the rat insulin II promoter (Ins2). |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
When using the BALB-RIPB7.1 mouse strain in a publication, please cite the originating article(s) and include JAX stock #005713 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrierfor Tg(Ins2-CD80)3B7Flv |
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