In these Cxcr6 knock-out/reporter mice EGFP expression is restricted to spleen and lymph nodes. They are suitable for use in applications related to studies of hepatitis and various immune responses.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Cxcr6 | chemokine (C-X-C motif) receptor 6 |
Mice homozygous for this EGFP "knock-in" are viable, fertile, normal in size, and do not display any behavioral abnormalities when maintained under barrier conditions. Lymph nodes and spleen show no endogenous gene expression. Lymphocytes from heterozygotes, but not homozygotes, show endogenous ligand binding. EGFP expression is restricted to spleen and lymph nodes, specifically activated/memory T cells, with a slightly higher intensity in homozygotes. Homozygous null mice show decreased EGFP+ CD1d-reactive NKT patrolling efficiency and decreased severity of induced acute hepatitis, while heterozygotes and wildtype mice show no differences. This mutant may be useful in studies of hepatitis, HIV, SIV, fluorescent T cell tracking, and various immune responses.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing an enhanced green fluorescent protein (EGFP)/SV40 poly(A)n cassette with a downstream loxP-flanked neomycin resistance gene and herpes simplex virus thymidine kinase was used to replace the endogenous coding sequence. The construct was electroporated into the 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase-expressing vector before being injected into C57BL/6 blastocysts. The resulting chimeric mice were bred to BALB/c mice. Heterozygotes were backcrossed to BALB/c mice for 12 generations before being made homozygous.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP expression mimics endogenous gene expression and is detected in the spleen and lymph nodes, specifically activated/memory T cells. |
Allele Name | targeted mutation 1, Daniel Littman |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Bonzo-; Bz-; cxcr6gfp; Cxcr6tm1Unu |
Gene Symbol and Name | Cxcr6, chemokine (C-X-C motif) receptor 6 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression mimics endogenous gene expression and is detected in the spleen and lymph nodes, specifically activated/memory T cells. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 9 |
Molecular Note | The coding sequence was replaced with an EGFP. RT-PCR failed to detect transcript in mutants. Strong GFP expression was detected in spleen and lymphoid nodes. |
Mutations Made By | Dr. Dan Littman, New York University Medical Center |
When maintaining a live colony, these mice are maintained as homozygotes.
When using the Bz- mouse strain in a publication, please cite the originating article(s) and include JAX stock #005700 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cxcr6<tm1Litt> |
Frozen Mouse Embryo | C.129P2-Cxcr6<tm1Litt>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.129P2-Cxcr6<tm1Litt>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.129P2-Cxcr6<tm1Litt>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C.129P2-Cxcr6<tm1Litt>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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