C.129P2-Cxcr6^tm1Litt/J

Stock number: 005700
Product name: Bz-
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

In these Cxcr6 knock-out/reporter mice EGFP expression is restricted to spleen and lymph nodes. They are suitable for use in applications related to studies of hepatitis and various immune responses.

Detailed description

Mice homozygous for this EGFP "knock-in" are viable, fertile, normal in size, and do not display any behavioral abnormalities when maintained under barrier conditions. Lymph nodes and spleen show no endogenous gene expression. Lymphocytes from heterozygotes, but not homozygotes, show endogenous ligand binding. EGFP expression is restricted to spleen and lymph nodes, specifically activated/memory T cells, with a slightly higher intensity in homozygotes. Homozygous null mice show decreased EGFP+ CD1d-reactive NKT patrolling efficiency and decreased severity of induced acute hepatitis, while heterozygotes and wildtype mice show no differences. This mutant may be useful in studies of hepatitis, HIV, SIV, fluorescent T cell tracking, and various immune responses.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

 

Development

A targeting vector containing an enhanced green fluorescent protein (EGFP)/SV40 poly(A)n cassette with a downstream loxP-flanked neomycin resistance gene and herpes simplex virus thymidine kinase was used to replace the endogenous coding sequence. The construct was electroporated into the 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase-expressing vector before being injected into C57BL/6 blastocysts. The resulting chimeric mice were bred to BALB/c mice. Heterozygotes were backcrossed to BALB/c mice for 12 generations before being made homozygous.

Allele

Symbol: Cxcr6^tm1Litt
Name: targeted mutation 1, Daniel Littman
MGI accession ID: MGI:3613526
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Cxcr6
Marker name: chemokine (C-X-C motif) receptor 6
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: EGFP expression mimics endogenous gene expression and is detected in the spleen and lymph nodes, specifically activated/memory T cells.
Molecular note: The coding sequence was replaced with an EGFP. RT-PCR failed to detect transcript in mutants. Strong GFP expression was detected in spleen and lymphoid nodes.