D1Lac.Cg-Tg(Tcra,Tcrb)24Efro/J

Stock number: 005694
Product name: TCR Tg
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These TCR Tg mice express a transgene containing the mouse alpha and beta chain of the T cell receptor recognizing both bovine and mouse type II collagen.

Detailed description

Transgenic mice are viable, fertile, and normal in size. This mutant carries a transgene for a fully functional bovine and mouse type II collagen (260-267) specific IA^q-restricted T cell receptor. Flow cytometric analysis show 90% Tcrb-V8.3 expression of CD4+ splenocytes and greater than 95% expression in CD4+ lymphocytes. The transgene confers severe accelerated autoimmune arthritis following type II collagen (CII) immunization. Splenocytes from tolerized mice have altered activation and memory cell surface markers and high levels of Th2 cytokine secretion compared to control. Estradiol completely prevents collagen-induced arthritis (CIA). This mutant mouse may be useful in studies of CIA, rheumatoid arthritis, and T regulatory cells and may also serve as a source of T cells that mediate development of autoimmune diseases.

Development

Transgenic constructs containing the mouse alpha and beta chain of the T cell receptor recognizing both bovine and mouse type II collagen (260-267) were cloned from the Tcra-V11.1,Tcra-J(J17) and Tcrb-V8.3,Tcrb-D(D1),Tcrb-J(J1.4) expressing I-A^q-restricted T cell hybridoma qCII85.33. The constructs were co-injected into pronuclei of (C57BL/6 x C3H)F1 eggs. PCR-positive mice (founder line 24) were backcrossed to DBA/1LacJ for 8 generations.

Allele

Symbol: Tg(Tcra,Tcrb)24Efro
Name: transgene insertion 24, Edward F Rosloniec
MGI accession ID: MGI:3707414
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Tcra,Tcrb)24Efro
Marker name: transgene insertion 24, Edward F Rosloniec
Chromosome: UN
Strain of origin: (C57BL/6 x C3H)F1
Expressed genes: Tcra,T cell receptor alpha chain,mouse, laboratory; Tcrb,T cell receptor beta chain,mouse, laboratory
Molecular note: Transgenic constructs containing the mouse alpha and beta chain of the T cell receptor recognizing both bovine and mouse type II collagen (260-267) were cloned from the Tcra-V11.1,Tcra-J(J17) and Tcrb-V8.3,Tcrb-D(D1),Tcrb-J(J1.4) expressing I-Aq-restricted T cell hybridoma qCII85.33. Line 24 was established. The transgenic T cell receptor is expressed on >90% of CD4+ T cells in the spleen and lymph nodes. >90% of alphabeta+ T cells in the thymus.