These Mx-Cre mice express a transgene containing cre recombinase directed by the Mx1 promoter.
Roberta Pelanda, National Jewish Medical and Research Ctr
Genetic Background | Generation |
---|---|
|
Allele Type |
---|
Transgenic (Recombinase-expressing, Inducible) |
The Cre recombinase is under the control of the Mx1 promoter. This promoter is silent in healthy mice, but can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA (such as poly I:C). When combined with a mutant carrying a gene that has been flanked by loxP recognition sites, the expression of Cre recombinase causes the flanked gene to be removed. This provides researchers with the capability to induce the "knockout" at any time during development. There is ~1% background recombination seen in mice not treated with interferon. The percent deletion of the targeted gene varies depending on tissue type, presumably due to the amount of interferon-responsive cells present or to the availability of interferon in each organ.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A transgenic construct containing the mouse Mx1 gene promoter, nuclear localization sequence-modified Cre recombinase and a 2.1 kb fragment from the human growth hormone gene (to provide the splicing and polyadenylation signals) was injected into (C57BL/6J X CBA/J)F2 fertilized eggs. The resulting transgenic mice were crossed to (C57BL/6J x 129Sv)F2 mice for an unknown number of generations. The mice were then backcrossed onto the BALB/cJ background for 10 generations.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
---|---|
Site of Expression | widespread pattern of expression; promoter induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA; provides capability to induce the "knockout" at any time during development |
Allele Name | transgene insertion 1, University of Cologne |
---|---|
Allele Type | Transgenic (Recombinase-expressing, Inducible) |
Allele Synonym(s) | Mx1cre; Mx1-Cre; MxCre; Mx-Cre; Mx-Cre 31; MxCreTg; Tg(Mx1-cre)hemi |
Gene Symbol and Name | Tg(Mx1-cre)1Cgn, transgene insertion 1, University of Cologne |
Gene Synonym(s) | |
Promoter | Mx1, MX dynamin-like GTPase 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | widespread pattern of expression; promoter induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA; provides capability to induce the "knockout" at any time during development |
Strain of Origin | (C57BL/6J x CBA/J)F2 |
Chromosome | 10 |
Molecular Note | This transgene expresses Cre recombinase under the control of an inducible Mx1 promoter, which is silent in healthy mice, and active in the liver and in lymphocytes after induction with IFN or pI-pC. The construct also contains a 2.1 kb fragment from the human growth hormone gene. |
Mutations Made By | Dr. Ralf Kuhn, Max-Delbrück Center for Molecular Medicine |
When maintaining a live colony, these mice are maintained as hemizygotes.
When using the Mx-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #005673 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Hemizygous or Non carrier for Tg(Mx1-cre)1Cgn |
Frozen Mouse Embryo | C.Cg-Tg(Mx1-cre)1Cgn/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.Cg-Tg(Mx1-cre)1Cgn/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | C.Cg-Tg(Mx1-cre)1Cgn/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | C.Cg-Tg(Mx1-cre)1Cgn/J Frozen Embryos | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.