ROSA26-rtTA mice can be used to generate triple mutant mice in which the tissue specificity of a cre-transgenic line and doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of a target gene.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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N7pN2F12
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), Reporter, Inducible, Transactivator) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the cre-transgenic line and doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing a loxP-flanked phosphoglycerate kinase-neomycin resistance gene-polyadenylation stop sequence (PGK-neo-pA) and a downstream reverse tetracycline-controlled transactivator gene-internal ribosomal entry site-enhanced green fluorescent protein gene-polyadenylation stop sequence (rtTA-IRES-EGFP-pA) fusion protein was inserted between exon 1 and 2 of the endogenous Gt(ROSA)26Sor locus. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into ICR blastocysts and the resulting chimeric males were backcrossed for germ-line transmission to ICR females. Heterozygous offspring were sent to The Jackson Laboratory (as Stock No. 005572). Upon arrival, some mice were backcrossed to C57BL/6J for at least 5 generations to generate this congenic strain (Stock No. 005670).
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | When these mutant mice are crossed to a strain expressing Cre recombinase, rtTA and EGFP are expressed in the cre-expressing tissues. These mice can be used to generate mutant mice in which the tissue specificity of cre-expression and doxycycline inducibility of the rtTA can be combined. |
Allele Name | targeted mutation 1, Andras Nagy |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Reporter, Inducible, Transactivator) |
Allele Synonym(s) | Gt(ROSA)26Sortm1(rtTA)Nagy; Gt(ROSA)26Sortm1Nagy; R26rtTA; Rosa26fs-rtTA; ROSA26-rtTA; ROSA26-rtTA-IRES-EGFP; Rosa-eGFP; Rosa-rtTALSL; rtTAflox |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | When these mutant mice are crossed to a strain expressing Cre recombinase, rtTA and EGFP are expressed in the cre-expressing tissues. These mice can be used to generate mutant mice in which the tissue specificity of cre-expression and doxycycline inducibility of the rtTA can be combined. |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 6 |
Molecular Note | A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. |
Mutations Made By | Gusztav Belteki and Jody Haigh, Mount Sinai Hospital |
Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred.
When using the ROSA26-rtTA-IRES-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #005670 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm1(rtTA,EGFP)Nagy> |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm1(rtTA EGFP)Nagy>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm1(rtTA EGFP)Nagy>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm1(rtTA EGFP)Nagy>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Gt(ROSA)26Sor<tm1(rtTA EGFP)Nagy>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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