This knock-out results in the complete ablation of the eosinophil lineage. This mutant may be useful for in vivo studies of eosinophil function and eosinophil-related pathologies, including asthma and pulmonary physiology.
Stuart H Orkin, Harvard University
Homozygous females and hemizygous males for the targeted mutation are viable, fertile, and normal in size. This mutation results in the complete ablation of the eosinophil lineage, even under conditions that normally stimulate eosinophil development, without affecting the development of other GATA-1 dependent lineages (erythroid, megakaryocytic, and mast cells). Expression of the endogenous gene is observed in erythroid and bone marrow cells. This mutant may be useful for in vivo studies of eosinophil function and eosinophil-related pathologies, including asthma and pulmonary physiology.
A targeting vector was designed to replace the double GATA-site 21 bp upstream of the first hematopoietic exon of the X-linked endogenous gene. This vector, containing a loxP-flanked phosphoglycerate kinase promoter driven neomycin resistance gene (PGK-neo), was electroporated into 129S1/Sv derived CJ-7 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a cre recombinase vector to remove the PGK-neo resulting in a mutant allele where the double GATA-site is replaced with a single loxP site. These cells were injected into C57BL/6 blastocysts and the resulting chimeric males were backcrossed to C57BL/6 females. Resultant heterozygotes were backcrossed to BALB/c for eight generations before being made homozygous/hemizygous.
|Allele Name||targeted mutation 6, Stuart Orkin|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||targeted mutation 6, Stuart Orkin; Gata1tm6Sho|
|Gene Symbol and Name||Gata1, GATA binding protein 1|
|Gene Synonym(s)||NFE1; NF-E1; XLTDA; XLANP; Gata-1; Gf-1; XLTT; ERYF1; GATA-1; GF-1; GF1; globin factor 1; Eryf1; Gf-1; Gata-1|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A high-affinity, palindromic "double" GATA protein binding site in the Gata1 promoter presumed to mediate positive Gata1 autoregulation was replaced by a floxed Pgk-neo cassette; transient Cre recombinase expression in ES cells left a single loxP site flanked by two Not1 sites. The 21-bp deleted segment comprised nucleotides -691 through -671 upstream of the last nucleotide of the first hematopoietically expressed exon of Gata1.|
|Mutations Made By|| |
Stuart Orkin, Harvard University
When maintaining a live colony, this colony is maintained as homozygotes.
When using the ΔdblGATA mouse strain in a publication, please cite the originating article(s) and include JAX stock #005653 in your Materials and Methods section.
|X linked - Heterozygous Females and Wild-type Males for Gata1<tm6Sho>, 1 pair minimum|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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